| Форма представления | Статьи в российских журналах и сборниках |
| Год публикации | 2024 |
| Язык | русский |
|
Ахатова Фарида Сериковна, автор
Баташева Светлана Николаевна, автор
Гурьянов Иван Дмитриевич, автор
Закирова Елена Юрьевна, автор
Науменко Екатерина Анатольевна, автор
|
| Библиографическое описание на языке оригинала |
Naumenko E. A. Fabrication and atomic force microscopy-based mechanical analysis of hepatic organoids / E. A. Naumenko, F. S. Akhatova, I. D. Guryanov [et al.] // Opera Medica et Physiologica. – 2024. – Vol. 11, No. 4. – P. 71-79. – DOI 10.24412/2500-2295-2024-4-71-79. |
| Аннотация |
Opera Medica et Physiologica |
| Ключевые слова |
HEPATIC ORGANOIDS, ATOMIC FORCE MICROSCOPY, FIBROSIS, EXTRACELLULAR MATRIX, MECHANICAL PROPERTIES |
| Название журнала |
Opera Medica et Physiologica
|
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=315892 |
| Файлы ресурса | |
|
|
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Ахатова Фарида Сериковна |
ru_RU |
| dc.contributor.author |
Баташева Светлана Николаевна |
ru_RU |
| dc.contributor.author |
Гурьянов Иван Дмитриевич |
ru_RU |
| dc.contributor.author |
Закирова Елена Юрьевна |
ru_RU |
| dc.contributor.author |
Науменко Екатерина Анатольевна |
ru_RU |
| dc.date.accessioned |
2024-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2024-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2024 |
ru_RU |
| dc.identifier.citation |
Naumenko E. A. Fabrication and atomic force microscopy-based mechanical analysis of hepatic organoids / E. A. Naumenko, F. S. Akhatova, I. D. Guryanov [et al.] // Opera Medica et Physiologica. – 2024. – Vol. 11, No. 4. – P. 71-79. – DOI 10.24412/2500-2295-2024-4-71-79. |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=315892 |
ru_RU |
| dc.description.abstract |
Opera Medica et Physiologica |
ru_RU |
| dc.description.abstract |
The liver is a vital organ involved in a wide range of processes such as detoxification, protein synthesis, metabolism, and hormone production. Liver diseases, both inherited and viral hepatitis, liver cancer and fatty degeneration are among the leading causes of death in the world. Recent advances in 3D cell culture technology include the use of pluripotent stem cells and adult stem cells that are cultured in vitro to form self-organizing systems. Organoids are self-organizing multicellular structures that reproduce the structure and function of organs and can be used to model the development, maintenance and repair of organs ex vivo. That is why the search for new methods for the formation of hepatic organoids seen as an urgent task. For the fabrication of 3D organoids we isolated hepatic duct cells from rat liver. The obtained cellular structures were analyzed using atomic force microscopy to estimate their mechanical properties and demonstrated increasing of Young Modulus in comparison with normal liver sections. Morphometric evaluation showed that extracellular matrix fibers occupy up to 60% of the organoid, while cell agglomerations up to 40% of it. We observed the spontaneously formed fibrotic liver tissue-like constructs within 21 days. So, obtained hepatic organoids characterized by a tissue-like structure with a predominance of extracellular matrix fibers in its composition similar to liver tissue affected by fibrosis. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
HEPATIC ORGANOIDS |
ru_RU |
| dc.subject |
ATOMIC FORCE MICROSCOPY |
ru_RU |
| dc.subject |
FIBROSIS |
ru_RU |
| dc.subject |
EXTRACELLULAR MATRIX |
ru_RU |
| dc.subject |
MECHANICAL PROPERTIES |
ru_RU |
| dc.title |
Fabrication and atomic force microscopy-based mechanical analysis of hepatic organoids |
ru_RU |
| dc.type |
Статьи в российских журналах и сборниках |
ru_RU |
|
EN 
中文 
ES 
Карта сайта
