КФУ. Карточка публикации. Rapid identification of Lactobacillus plantarum by RAPD-PCR

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ФЕДЕРАЛЬНЫЙ УНИВЕРСИТЕТ

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RAPID IDENTIFICATION OF LACTOBACILLUS PLANTARUM BY RAPD-PCR

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

1998

Язык

русский

Авторы, сотрудники КФУ

Валидов Шамиль Завдатович, автор

Библиографическое описание на языке оригинала

Validov, S. Identification, Lactobacillus plantarum, Monitoring, RAPD-PCR, Silage, Species / Pan'kova, N.V., Kozlova, E.V., Kuz'min, N.P., Klimenko, V.V., Boronin, A.M Microbiology. – 1998. 68- №3. – С. 317 - 322. – Библиогр. : с. 317.

Аннотация

The wide distribution and practical use of lactobacilli necessitates development of methods for their rapid isolation and identification. In particular, such methods are necessary for monitoring natural and introduced strains of lactobacilli during ensilage to optimize this process. Twelve strains of lactobacilli were isolated from silage and wheat rhizosphere on Rogosa medium. The polymerase chain reaction with arbitrary primer (random amplified polymorphic DNA polymerase chain reaction, RAPD-PCR) was used for species identification of the isolates and collection strains. A 17-nucleotide-long primer designated pUC/M13 was used. The patterns of amplification products often isolates were found to be similar to those of the collection strains of Lactobacillus plantarum, indicating that they belong to this species. A study of physiological and biochemical characteristics (growth at different temperatures and the profile of fermented carbohydrates) confirmed conclusions based on the result

Ключевые слова

Identification, Lactobacillus plantarum, Monitoring, RAPD-PCR, Silage, Species

Название журнала

Микробиология

URL

http://www.scopus.com/source/sourceInfo.url?sourceId=20269&origin=recordpage

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https://repository.kpfu.ru/?p_id=80361

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Валидов Шамиль Завдатович	ru_RU
dc.date.accessioned	1998-01-01T00:00:00Z	ru_RU
dc.date.available	1998-01-01T00:00:00Z	ru_RU
dc.date.issued	1998	ru_RU
dc.identifier.citation	Validov, S. Identification, Lactobacillus plantarum, Monitoring, RAPD-PCR, Silage, Species / Pan'kova, N.V., Kozlova, E.V., Kuz'min, N.P., Klimenko, V.V., Boronin, A.M Microbiology. – 1998. 68- №3. – С. 317 - 322. – Библиогр. : с. 317.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=80361	ru_RU
dc.description.abstract	Микробиология	ru_RU
dc.description.abstract	The wide distribution and practical use of lactobacilli necessitates development of methods for their rapid isolation and identification. In particular, such methods are necessary for monitoring natural and introduced strains of lactobacilli during ensilage to optimize this process. Twelve strains of lactobacilli were isolated from silage and wheat rhizosphere on Rogosa medium. The polymerase chain reaction with arbitrary primer (random amplified polymorphic DNA polymerase chain reaction, RAPD-PCR) was used for species identification of the isolates and collection strains. A 17-nucleotide-long primer designated pUC/M13 was used. The patterns of amplification products often isolates were found to be similar to those of the collection strains of Lactobacillus plantarum, indicating that they belong to this species. A study of physiological and biochemical characteristics (growth at different temperatures and the profile of fermented carbohydrates) confirmed conclusions based on the result	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Identification	ru_RU
dc.subject	Lactobacillus plantarum	ru_RU
dc.subject	Monitoring	ru_RU
dc.subject	RAPD-PCR	ru_RU
dc.subject	Silage	ru_RU
dc.subject	Species	ru_RU
dc.title	Rapid identification of Lactobacillus plantarum by RAPD-PCR	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU