КФУ. Карточка публикации. Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies

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GENETIC TOOLS FOR TAGGING GRAM-NEGATIVE BACTERIA WITH MCHERRY FOR VISUALIZATION IN VITRO AND IN NATURAL HABITATS, BIOFILM AND PATHOGENICITY STUDIES

Форма представления

Статьи в зарубежных журналах и сборниках

Год публикации

2011

Язык

английский

Авторы, сотрудники КФУ

Валидов Шамиль Завдатович, автор

Библиографическое описание на языке оригинала

Lagendijk EL, Validov S, Lamers GEM, de Weert S, Bloemberg GV. 2010. Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies. Fems Microbiology Letters 305:81-90.

Аннотация

Live-cell imaging techniques are essential to gain a better understanding of microbial functioning in natural systems, for example in biofilms. Autofluorescent proteins, such as the green fluorescent protein (GFP) and the red fluorescent protein (DsRed), are valuable tools for studying microbial communities in their natural environment. Because of the functional limitations of DsRed such as slow maturation and low photostability, new and improved variants were created such as mCherry. In this study, we developed genetic tools for labeling Gram-negative bacteria in order to visualize them in vitro and in their natural environment without the necessity of antibiotic pressure for maintenance. mcherry was cloned into two broad host-range cloning vectors and a pBK-miniTn7 transposon under the constitutive expression of the tac promoter. The applicability of the different constructs was shown in Escherichia coli, various Pseudomonas spp. and Edwardsiella tarda. The expression of mcherry was

Ключевые слова

Pseudomonas, Live-cell imaging

Название журнала

FEMS Microbiology Letters

URL

http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2010.01916.x/pdf

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https://repository.kpfu.ru/?p_id=94463

Полная запись метаданных

Поле DC	Значение	Язык
dc.contributor.author	Валидов Шамиль Завдатович	ru_RU
dc.date.accessioned	2011-01-01T00:00:00Z	ru_RU
dc.date.available	2011-01-01T00:00:00Z	ru_RU
dc.date.issued	2011	ru_RU
dc.identifier.citation	Lagendijk EL, Validov S, Lamers GEM, de Weert S, Bloemberg GV. 2010. Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies. Fems Microbiology Letters 305:81-90.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/?p_id=94463	ru_RU
dc.description.abstract	FEMS Microbiology Letters	ru_RU
dc.description.abstract	Live-cell imaging techniques are essential to gain a better understanding of microbial functioning in natural systems, for example in biofilms. Autofluorescent proteins, such as the green fluorescent protein (GFP) and the red fluorescent protein (DsRed), are valuable tools for studying microbial communities in their natural environment. Because of the functional limitations of DsRed such as slow maturation and low photostability, new and improved variants were created such as mCherry. In this study, we developed genetic tools for labeling Gram-negative bacteria in order to visualize them in vitro and in their natural environment without the necessity of antibiotic pressure for maintenance. mcherry was cloned into two broad host-range cloning vectors and a pBK-miniTn7 transposon under the constitutive expression of the tac promoter. The applicability of the different constructs was shown in Escherichia coli, various Pseudomonas spp. and Edwardsiella tarda. The expression of mcherry was	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Pseudomonas	ru_RU
dc.subject	Live-cell imaging	ru_RU
dc.title	Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies	ru_RU
dc.type	Статьи в зарубежных журналах и сборниках	ru_RU