Казанский (Приволжский) федеральный университет, КФУ
КАЗАНСКИЙ
ФЕДЕРАЛЬНЫЙ УНИВЕРСИТЕТ
 
GENETIC TOOLS FOR TAGGING GRAM-NEGATIVE BACTERIA WITH MCHERRY FOR VISUALIZATION IN VITRO AND IN NATURAL HABITATS, BIOFILM AND PATHOGENICITY STUDIES
Форма представленияСтатьи в зарубежных журналах и сборниках
Год публикации2011
Языканглийский
  • Валидов Шамиль Завдатович, автор
  • Библиографическое описание на языке оригинала Lagendijk EL, Validov S, Lamers GEM, de Weert S, Bloemberg GV. 2010. Genetic tools for tagging Gram-negative bacteria with mCherry for visualization in vitro and in natural habitats, biofilm and pathogenicity studies. Fems Microbiology Letters 305:81-90.
    Аннотация Live-cell imaging techniques are essential to gain a better understanding of microbial functioning in natural systems, for example in biofilms. Autofluorescent proteins, such as the green fluorescent protein (GFP) and the red fluorescent protein (DsRed), are valuable tools for studying microbial communities in their natural environment. Because of the functional limitations of DsRed such as slow maturation and low photostability, new and improved variants were created such as mCherry. In this study, we developed genetic tools for labeling Gram-negative bacteria in order to visualize them in vitro and in their natural environment without the necessity of antibiotic pressure for maintenance. mcherry was cloned into two broad host-range cloning vectors and a pBK-miniTn7 transposon under the constitutive expression of the tac promoter. The applicability of the different constructs was shown in Escherichia coli, various Pseudomonas spp. and Edwardsiella tarda. The expression of mcherry was
    Ключевые слова Pseudomonas, Live-cell imaging
    Название журнала FEMS Microbiology Letters
    URL http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2010.01916.x/pdf
    Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку https://repository.kpfu.ru/?p_id=94463

    Полная запись метаданных