| Форма представления | Статьи в российских журналах и сборниках |
| Год публикации | 2015 |
| Язык | английский |
|
Абдрахимова Йолдыз Раисовна, автор
|
|
Андреев Игорь Михайлович, автор
Шугаев Александр Григорьевич, автор
|
| Библиографическое описание на языке оригинала |
Abdrakhimova Y.R., I.M.Andreev,A.G.Shugaev.Determination of ROS Generation Rates in Plant Mitochondria in vitro Using Fluorescent Indicators: Non-Specific Effects
of Inhibitors of Terminal Oxidases//Russian Journal of Plant Physiology, 2015, Vol. 62, No. 1, pp. 136-141.
DOI: 10.1134/S1021443714060028 |
| Аннотация |
A possibility of quantitative analysis of the ROS production by mitochondria isolated from etiolated winter wheat seedlings was studied by following hydrogen peroxide accumulation in medium of organelle incubation using a new highly sensitive test-system including the fluorogenic indicator Amplex Red and horseradish peroxidase. Experimental evidence is presented indicating that the classical inhibitors of the terminal oxidases of the plant mitochondrial respiratory chain can inactivate the ROS detecting test-systems in vitro because of interactions with
their functional components. These results are discussed
in comparison with literature data obtained by similar test-systems and indicate that there are considerable limitations for obtaining reliable experimental evidence of the antioxidant role of alternative cyanide-resistant oxidase in plant mitochondria. |
| Ключевые слова |
Triticum aestivum, mitochondria, reactive oxygen species, fluorescent indicators, Amplex Red,
alamethicin |
| Название журнала |
Russian Journal of Plant Physiology
|
| URL |
http://link.springer.com/article/10.1134/S1021443714060028 |
| Пожалуйста, используйте этот идентификатор, чтобы цитировать или ссылаться на эту карточку |
https://repository.kpfu.ru/?p_id=98432 |
| Файлы ресурса | |
|
|
Полная запись метаданных  |
| Поле DC |
Значение |
Язык |
| dc.contributor.author |
Абдрахимова Йолдыз Раисовна |
ru_RU |
| dc.contributor.author |
Андреев Игорь Михайлович |
ru_RU |
| dc.contributor.author |
Шугаев Александр Григорьевич |
ru_RU |
| dc.date.accessioned |
2015-01-01T00:00:00Z |
ru_RU |
| dc.date.available |
2015-01-01T00:00:00Z |
ru_RU |
| dc.date.issued |
2015 |
ru_RU |
| dc.identifier.citation |
Abdrakhimova Y.R., I.M.Andreev,A.G.Shugaev.Determination of ROS Generation Rates in Plant Mitochondria in vitro Using Fluorescent Indicators: Non-Specific Effects
of Inhibitors of Terminal Oxidases//Russian Journal of Plant Physiology, 2015, Vol. 62, No. 1, pp. 136-141.
DOI: 10.1134/S1021443714060028 |
ru_RU |
| dc.identifier.uri |
https://repository.kpfu.ru/?p_id=98432 |
ru_RU |
| dc.description.abstract |
Russian Journal of Plant Physiology |
ru_RU |
| dc.description.abstract |
A possibility of quantitative analysis of the ROS production by mitochondria isolated from etiolated winter wheat seedlings was studied by following hydrogen peroxide accumulation in medium of organelle incubation using a new highly sensitive test-system including the fluorogenic indicator Amplex Red and horseradish peroxidase. Experimental evidence is presented indicating that the classical inhibitors of the terminal oxidases of the plant mitochondrial respiratory chain can inactivate the ROS detecting test-systems in vitro because of interactions with
their functional components. These results are discussed
in comparison with literature data obtained by similar test-systems and indicate that there are considerable limitations for obtaining reliable experimental evidence of the antioxidant role of alternative cyanide-resistant oxidase in plant mitochondria. |
ru_RU |
| dc.language.iso |
ru |
ru_RU |
| dc.subject |
Triticum aestivum |
ru_RU |
| dc.subject |
mitochondria |
ru_RU |
| dc.subject |
reactive oxygen species |
ru_RU |
| dc.subject |
fluorescent indicators |
ru_RU |
| dc.subject |
Amplex Red |
ru_RU |
| dc.subject |
alamethicin |
ru_RU |
| dc.title |
Determination of ROS Generation Rates in Plant Mitochondria in vitro Using Fluorescent Indicators: Non-Specific Effects
of Inhibitors of Terminal Oxidases |
ru_RU |
| dc.type |
Статьи в российских журналах и сборниках |
ru_RU |
|
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