KFU. Card publication. Expression of Pantoea agglomerans phytase from a strong constitutive promoter in Arabidopsis thaliana plants

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EXPRESSION OF PANTOEA AGGLOMERANS PHYTASE FROM A STRONG CONSTITUTIVE PROMOTER IN ARABIDOPSIS THALIANA PLANTS

Form of presentation

Articles in international journals and collections

Year of publication

2015

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Authors, employees of KFU

Bulmakova Darya Sergeevna, author

Valeeva Liya Rashitovna, author

Nyamsuren Chuluunceceg ., author

Shakirov Evgeniy Vitalevich, author

Sharipova Margarita Rashidovna, author

Authors, postgraduates KFU

Valeeva Liya Rashitovna, postgraduate kfu

Bibliographic description in the original language

Valeeva, L. R. Expression of Pantoea agglomerans phytase from a strong constitutive promoter in Arabidopsis thaliana plants / L. R Valeeva, Ch. Nyamsuren, D. S Troshagina, M. R Sharipova, E. V. Shakirov // RJPBCS. - 2015. - V. 6(4). - P.99-104.

Annotation

In this study we report construction of an efficient gene expression system in plants and subsequent characterization of transgenic Arabidopsis thaliana expressing a bacterial paPhyC phytase gene from Pantoea sp. Phytase gene expression is controlled by a strong 35S constitutive promoter from cauliflower mosaic virus. All identified transgenic plants had multiple T-DNA insertions in the genome. Expression of paPhyC phytase mRNA in plant tissue was confirmed by RT-PCR in the second generation of transgenic plants, and phytase protein expression was confirmed by Western blotting. Our data indicate that bacterial phytase expression in plants can be an efficient way to potentially increase crop performance in conditions of inorganic phosphorus deficiency in the soil.

Keywords

phytase Pantoea sp., recombinant protein expression in plants, CaMV35S promoter, transgenic plants, phosphorus deficiency, Arabidopsis thaliana

The name of the journal

Research Journal of Pharmaceutical, Biological and Chemical Sciences

URL

http://www.rjpbcs.com/pdf/2015_6(4)/[17].pdf

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=125195&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Bulmakova Darya Sergeevna	ru_RU
dc.contributor.author	Valeeva Liya Rashitovna	ru_RU
dc.contributor.author	Nyamsuren Chuluunceceg .	ru_RU
dc.contributor.author	Shakirov Evgeniy Vitalevich	ru_RU
dc.contributor.author	Sharipova Margarita Rashidovna	ru_RU
dc.contributor.author	Valeeva Liya Rashitovna	ru_RU
dc.date.accessioned	2015-01-01T00:00:00Z	ru_RU
dc.date.available	2015-01-01T00:00:00Z	ru_RU
dc.date.issued	2015	ru_RU
dc.identifier.citation	Valeeva, L. R. Expression of Pantoea agglomerans phytase from a strong constitutive promoter in Arabidopsis thaliana plants / L. R Valeeva, Ch. Nyamsuren, D. S Troshagina, M. R Sharipova, E. V. Shakirov // RJPBCS. - 2015. - V. 6(4). - P.99-104.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=125195&p_lang=2	ru_RU
dc.description.abstract	Research Journal of Pharmaceutical, Biological and Chemical Sciences	ru_RU
dc.description.abstract	In this study we report construction of an efficient gene expression system in plants and subsequent characterization of transgenic Arabidopsis thaliana expressing a bacterial paPhyC phytase gene from Pantoea sp. Phytase gene expression is controlled by a strong 35S constitutive promoter from cauliflower mosaic virus. All identified transgenic plants had multiple T-DNA insertions in the genome. Expression of paPhyC phytase mRNA in plant tissue was confirmed by RT-PCR in the second generation of transgenic plants, and phytase protein expression was confirmed by Western blotting. Our data indicate that bacterial phytase expression in plants can be an efficient way to potentially increase crop performance in conditions of inorganic phosphorus deficiency in the soil.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	phytase Pantoea sp.	ru_RU
dc.subject	recombinant protein expression in plants	ru_RU
dc.subject	CaMV35S promoter	ru_RU
dc.subject	transgenic plants	ru_RU
dc.subject	phosphorus deficiency	ru_RU
dc.subject	Arabidopsis thaliana	ru_RU
dc.title	Expression of Pantoea agglomerans phytase from a strong constitutive promoter in Arabidopsis thaliana plants	ru_RU
dc.type	Articles in international journals and collections	ru_RU