KFU. Card publication. Complex electrochemical and impedimetric evaluation of DNA damage by using DNA biosensor based on a carbon screen-printed electrode

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COMPLEX ELECTROCHEMICAL AND IMPEDIMETRIC EVALUATION OF DNA DAMAGE BY USING DNA BIOSENSOR BASED ON A CARBON SCREEN-PRINTED ELECTRODE

Form of presentation

Articles in international journals and collections

Year of publication

2011

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Authors, employees of KFU

Ziyatdinova Guzel Kamilevna, author

Other authors

Jan Labuda , author

Bibliographic description in the original language

Ziyatdinova, G. Complex electrochemical and impedimetric evaluation of DNA damage by using DNA biosensor based on a carbon screen-printed electrode / G. Ziyatdinova, J. Labuda // Anal. Methods. - 2011. - V. 3. - No. 12. - P. 2777-2782.

Annotation

DNA biosensor (DNA/SWCNT-COOH-CHIT/SPCE) composed of dsDNA adsorptive layer on a carboxylated single-walled carbon nanotubes?chitosan composite deposited at a commercial carbon based screen-printed electrode has been prepared and applied to a complex investigation of damage to DNA by the Fenton type cleavage agent (hydroxyl radicals formed in the mixture of Cu2+, H2O2 and ascorbic acid) and copper(II)?quercetin system in 0.1 M PBS pH 7.0 under aerobic conditions. The dsDNA damage detection is performed by using square-wave voltammetry (SWV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in 1x10(-7) M thioridazine and 1x10(-3) M K4[Fe(CN)6]/K3Fe(CN)6 in the 0.1 M phosphate buffer solution, pH 7.0. Initial enhancement of the intrinsic guanine and adenine moieties SWV response indicates opening of the helix structure in the first stage of damage. Decrease in the intercalated thioridazine response confirms damage of the helix structure and deep DNA degradation.

Keywords

Biosensors, DNA damage, Electrochemistry

The name of the journal

Analytical Methods

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-82555169343&partnerID=40&md5=2e12dfb4b477d73245f14e4292a3892b

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=134859&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Ziyatdinova Guzel Kamilevna	ru_RU
dc.contributor.author	Jan Labuda	ru_RU
dc.date.accessioned	2011-01-01T00:00:00Z	ru_RU
dc.date.available	2011-01-01T00:00:00Z	ru_RU
dc.date.issued	2011	ru_RU
dc.identifier.citation	Ziyatdinova, G. Complex electrochemical and impedimetric evaluation of DNA damage by using DNA biosensor based on a carbon screen-printed electrode / G. Ziyatdinova, J. Labuda // Anal. Methods. - 2011. - V. 3. - No. 12. - P. 2777-2782.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=134859&p_lang=2	ru_RU
dc.description.abstract	Analytical Methods	ru_RU
dc.description.abstract	DNA biosensor (DNA/SWCNT-COOH-CHIT/SPCE) composed of dsDNA adsorptive layer on a carboxylated single-walled carbon nanotubes?chitosan composite deposited at a commercial carbon based screen-printed electrode has been prepared and applied to a complex investigation of damage to DNA by the Fenton type cleavage agent (hydroxyl radicals formed in the mixture of Cu2+, H2O2 and ascorbic acid) and copper(II)?quercetin system in 0.1 M PBS pH 7.0 under aerobic conditions. The dsDNA damage detection is performed by using square-wave voltammetry (SWV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in 1x10(-7) M thioridazine and 1x10(-3) M K4[Fe(CN)6]/K3Fe(CN)6 in the 0.1 M phosphate buffer solution, pH 7.0. Initial enhancement of the intrinsic guanine and adenine moieties SWV response indicates opening of the helix structure in the first stage of damage. Decrease in the intercalated thioridazine response confirms damage of the helix structure and deep DNA degradation.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Biosensors	ru_RU
dc.subject	DNA damage	ru_RU
dc.subject	Electrochemistry	ru_RU
dc.title	Complex electrochemical and impedimetric evaluation of DNA damage by using DNA biosensor based on a carbon screen-printed electrode	ru_RU
dc.type	Articles in international journals and collections	ru_RU