KFU. Card publication. Disposable electrochemical biosensor with multiwalled carbon nanotubes - Chitosan composite layer for the detection of deep DNA damage

- RUS
- 中文
- ES

DISPOSABLE ELECTROCHEMICAL BIOSENSOR WITH MULTIWALLED CARBON NANOTUBES - CHITOSAN COMPOSITE LAYER FOR THE DETECTION OF DEEP DNA DAMAGE

Form of presentation

Articles in international journals and collections

Year of publication

2008

��

��

Authors, employees of KFU

Ziyatdinova Guzel Kamilevna, author

Other authors

Galandova Julia , author

Labuda Jan , author

Bibliographic description in the original language

Galandova, J.Disposable electrochemical biosensor with multiwalled carbon nanotubes - Chitosan composite layer for the detection of deep DNA damage / J. Galandova, G. Ziyatdinova, J. Labuda // Anal. Sci. - 2008. - V. 24. - No. 6. - P. 711-716.

Annotation

A novel electrochemical DNA-based biosensor for the detection of deep DNA damage was designed employing the bionanocomposite layer of multiwalled carbon nanotubes (MWNT) in chitosan (CHIT) deposited on a screen printed carbon electrode (SPCE). The biocomponent represented by double-stranded (ds) herring sperm DNA was immobilized on this composite using layer-by-layer coverage to form a robust film. Individual and complex electrode modifiers are characterized by a differential pulse voltammetry (DPV) with the DNA redox marker [Co(phen)3]3+, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with [Fe(CN)6]3? as a redox probe in a phosphate buffer solution (PBS). A good correlation between the CV and EIS parameters has been found. Differences between the CV and EIS signals of the electrodes without and with DNA are used to detect deep damage to DNA, advantageously using simple working procedures in the same experiment.

Keywords

Electrochemical impedance spectroscopy, multiwalled carbon nanotubes, chitosan, DNA electrochemical biosensor, DNA damage

The name of the journal

Analytical Sciences

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-49749146402&partnerID=40&md5=a53949e16b8cc8da0efec92020659503

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=134867&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Ziyatdinova Guzel Kamilevna	ru_RU
dc.contributor.author	Galandova Julia	ru_RU
dc.contributor.author	Labuda Jan	ru_RU
dc.date.accessioned	2008-01-01T00:00:00Z	ru_RU
dc.date.available	2008-01-01T00:00:00Z	ru_RU
dc.date.issued	2008	ru_RU
dc.identifier.citation	Galandova, J.Disposable electrochemical biosensor with multiwalled carbon nanotubes - Chitosan composite layer for the detection of deep DNA damage / J. Galandova, G. Ziyatdinova, J. Labuda // Anal. Sci. - 2008. - V. 24. - No. 6. - P. 711-716.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=134867&p_lang=2	ru_RU
dc.description.abstract	Analytical Sciences	ru_RU
dc.description.abstract	A novel electrochemical DNA-based biosensor for the detection of deep DNA damage was designed employing the bionanocomposite layer of multiwalled carbon nanotubes (MWNT) in chitosan (CHIT) deposited on a screen printed carbon electrode (SPCE). The biocomponent represented by double-stranded (ds) herring sperm DNA was immobilized on this composite using layer-by-layer coverage to form a robust film. Individual and complex electrode modifiers are characterized by a differential pulse voltammetry (DPV) with the DNA redox marker [Co(phen)3]3+, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with [Fe(CN)6]3? as a redox probe in a phosphate buffer solution (PBS). A good correlation between the CV and EIS parameters has been found. Differences between the CV and EIS signals of the electrodes without and with DNA are used to detect deep damage to DNA, advantageously using simple working procedures in the same experiment.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Electrochemical impedance spectroscopy	ru_RU
dc.subject	multiwalled carbon nanotubes	ru_RU
dc.subject	chitosan	ru_RU
dc.subject	DNA electrochemical biosensor	ru_RU
dc.subject	DNA damage	ru_RU
dc.title	Disposable electrochemical biosensor with multiwalled carbon nanotubes - Chitosan composite layer for the detection of deep DNA damage	ru_RU
dc.type	Articles in international journals and collections	ru_RU