KFU. Card publication. Electrochemical determination of the total antioxidant capacity of human plasma

KAZAN
FEDERAL UNIVERSITY

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ELECTROCHEMICAL DETERMINATION OF THE TOTAL ANTIOXIDANT CAPACITY OF HUMAN PLASMA

Form of presentation

Articles in international journals and collections

Year of publication

2005

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Authors, employees of KFU

Budnikov German Konstantinovich, author

Ziyatdinova Guzel Kamilevna, author

Other authors

Pogorelcev Valeriy Ilich, author

Bibliographic description in the original language

Ziyatdinova G.K., Budnikov H.C., Pogorel'tzev V.I. Electrochemical determination of the total antioxidant capacity of human plasma // Anal. Bioanal. Chem. - 2005. - V. 381. - No. 8. - P. 1546-1551.

Annotation

Electrochemical reduction of oxygen at glassy carbon electrode in 0.05 M solution of (C2H5)4NI in dimethylformamide leads to generation of superoxide anion-radical. Product of one-electron reversible oxygen reduction (superoxide anion-radical) reacts with antioxidants. Process is based on the protonation of the anion-radical with antioxidant. Rate constants of this interaction were calculated. Human plasma antioxidants also react with electrochemically generated superoxide anion-radical. The voltammetric method for estimation of plasma?s total antioxidant capacity (TAC) based on this reaction is proposed. Moreover, TAC of plasma was determined using constant-current coulometry with the electrogenerated bromine as active species. There is correlation between TAC data received by voltammetry (�2?- with α-tocopherol units) and coulometry (Br2 as titrant). TAC of plasma from patients with purulent infections was determined.

Keywords

Total antioxidant capacity, voltammetry, superoxide anion-radical, plasma

The name of the journal

Analytical and Bioanalytical Chemistry

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-18144393804&partnerID=40&md5=6077157cadac2ea2f4e23d9b7d5e9e03

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=134872&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Budnikov German Konstantinovich	ru_RU
dc.contributor.author	Ziyatdinova Guzel Kamilevna	ru_RU
dc.contributor.author	Pogorelcev Valeriy Ilich	ru_RU
dc.date.accessioned	2005-01-01T00:00:00Z	ru_RU
dc.date.available	2005-01-01T00:00:00Z	ru_RU
dc.date.issued	2005	ru_RU
dc.identifier.citation	Ziyatdinova G.K., Budnikov H.C., Pogorel'tzev V.I. Electrochemical determination of the total antioxidant capacity of human plasma // Anal. Bioanal. Chem. - 2005. - V. 381. - No. 8. - P. 1546-1551.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=134872&p_lang=2	ru_RU
dc.description.abstract	Analytical and Bioanalytical Chemistry	ru_RU
dc.description.abstract	Electrochemical reduction of oxygen at glassy carbon electrode in 0.05 M solution of (C2H5)4NI in dimethylformamide leads to generation of superoxide anion-radical. Product of one-electron reversible oxygen reduction (superoxide anion-radical) reacts with antioxidants. Process is based on the protonation of the anion-radical with antioxidant. Rate constants of this interaction were calculated. Human plasma antioxidants also react with electrochemically generated superoxide anion-radical. The voltammetric method for estimation of plasma?s total antioxidant capacity (TAC) based on this reaction is proposed. Moreover, TAC of plasma was determined using constant-current coulometry with the electrogenerated bromine as active species. There is correlation between TAC data received by voltammetry (�2?- with α-tocopherol units) and coulometry (Br2 as titrant). TAC of plasma from patients with purulent infections was determined.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Total antioxidant capacity	ru_RU
dc.subject	voltammetry	ru_RU
dc.subject	superoxide anion-radical	ru_RU
dc.subject	plasma	ru_RU
dc.title	Electrochemical determination of the total antioxidant capacity of human plasma	ru_RU
dc.type	Articles in international journals and collections	ru_RU