KFU. Card publication. Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons

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OPTOGENETIC APPROACH FOR INVESTIGATION OF EXCITATORY AND INHIBITORY NETWORK GABA ACTIONS IN MICE EXPRESSING CHANNELRHODOPSIN-2 IN GABAERGIC NEURONS

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Khazipov Rustem Narimanovich, author

Other authors

Baude Agnes , author

Tressard Thomas , author

Bibliographic description in the original language

Valeeva G. An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons / G. Valeeva, T. Tressard, M. Mukhtarov, A. Baude, R. Khazipov // J Neurosci. - Vol. 36. - � 22. - R. 5961-5973.

Annotation

To investigate excitatory and inhibitory GABA actions in cortical neuronal networks, we present a novel optogenetic approach using a mouse knock-in line with conditional expression of channelrhodopsin-2 (ChR2) in GABAergic interneurons. During whole-cell recordings from hippocampal and neocortical slices from postnatal day (P) 2?P15 mice, photostimulation caused depolarization and excitation of interneurons and evoked barrages of postsynaptic GABAergic currents. Excitatory/inhibitory GABA actions on pyramidal cells were assessed by monitoring the alteration in the frequency of EPSCs during photostimulation of interneurons. We found that in slices from P2?P8 mice, photostimulation evoked an increase in EPSC frequency, whereas in P9 ?P15 mice the response switched to a reduction in EPSC frequency, indicating a developmental excitatory-to-inhibitory switch in GABA actions on glutamatergic neurons. Using a similar approach in urethane-anesthetized animals in vivo, we found that phot

Keywords

ChR2, cortex, GABA, hippocampus, inhibition, interneurons, patch clamp, optogenetics

The name of the journal

J NEUROSCI

URL

http://www.jneurosci.org/content/36/22/5961.long

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=135715&p_lang=2

Resource files

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Valeeva_et_al.__2016.pdf	3,43	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Khazipov Rustem Narimanovich	ru_RU
dc.contributor.author	Baude Agnes	ru_RU
dc.contributor.author	Tressard Thomas	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Valeeva G. An Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons / G. Valeeva, �. Tressard, �. Mukhtarov, �. Baude, R. Khazipov // J Neurosci. - Vol. 36. - � 22. - �. 5961-5973.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=135715&p_lang=2	ru_RU
dc.description.abstract	J NEUROSCI	ru_RU
dc.description.abstract	To investigate excitatory and inhibitory GABA actions in cortical neuronal networks, we present a novel optogenetic approach using a mouse knock-in line with conditional expression of channelrhodopsin-2 (ChR2) in GABAergic interneurons. During whole-cell recordings from hippocampal and neocortical slices from postnatal day (P) 2?P15 mice, photostimulation caused depolarization and excitation of interneurons and evoked barrages of postsynaptic GABAergic currents. Excitatory/inhibitory GABA actions on pyramidal cells were assessed by monitoring the alteration in the frequency of EPSCs during photostimulation of interneurons. We found that in slices from P2?P8 mice, photostimulation evoked an increase in EPSC frequency, whereas in P9 ?P15 mice the response switched to a reduction in EPSC frequency, indicating a developmental excitatory-to-inhibitory switch in GABA actions on glutamatergic neurons. Using a similar approach in urethane-anesthetized animals in vivo, we found that phot	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	ChR2	ru_RU
dc.subject	cortex	ru_RU
dc.subject	GABA	ru_RU
dc.subject	hippocampus	ru_RU
dc.subject	inhibition	ru_RU
dc.subject	interneurons	ru_RU
dc.subject	patch clamp	ru_RU
dc.subject	optogenetics	ru_RU
dc.title	Optogenetic Approach for Investigation of Excitatory and Inhibitory Network GABA Actions in Mice Expressing Channelrhodopsin-2 in GABAergic Neurons	ru_RU
dc.type	Articles in international journals and collections	ru_RU