KFU. Card publication. Recombinant FtsZ Proteins from Mollicutes Interactwith Escherichia coli Division Machinery

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RECOMBINANT FTSZ PROTEINS FROM MOLLICUTES INTERACTWITH ESCHERICHIA COLI DIVISION MACHINERY

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Kayumov Ayrat Rashitovich, author

Other authors

Vedyakin Aleksey , author

Vishnyakov Innokentiy , author

Sabancev Anton Vladimirovich, author

Khodorkovskiy Mikhail Alekseevich, author

Bibliographic description in the original language

Vedyaykin A.D. Recombinant FtsZ Proteins from Mollicutes Interactwith Escherichia coli Division Machinery / A.D. Vedyaykin, A.V. Sabantsev, M.A. Khodorkovskii, A.R. Kayumov, I.E. Vishnyakov // BioNanoScience. - 2016. DOI: 10.1007/s12668-016-0248-3.

Annotation

FtsZ is a well-known prokaryotic tubulin homo-logue. It participates in cytokinesis in most bacteria and isconsidered to be a key division protein. However, the ge-nomes of some mycoplasma species (class Mollicutes) lackof the ftsZ genes or even ftsZ-like genes. Moreover, it wasdemonstrated for Myco plasma mycoides and Mycoplasmagenitalium that FtsZ protein is not essential for division andsurvival. In this study, we induced an expression of recombi-nant FtsZ proteins from Mollicutes Acholeplasma laidlawiiand Mycoplasma gallisepticum in Escherichia coli cells inthe background of normal expression of its own FtsZ. Byusing indirect doub le immunofluo rescence in combinationwith single-molecule localization microscopy, it was demon-strated that both mycoplasmal FtsZ proteins are able to inter-fere with E. coli division machinery, but the interaction differsdepending on the protein used. Our data suggest that FtsZproteins from Mollicutes may play an important role in thecell division of co

Keywords

Cell division, FtsZ, Mycoplasma, Escherichia coli

The name of the journal

BioNanoScience

URL

http://www.readcube.com/articles/10.1007/s12668-016-0248-3?author_access_token=dIhAaPQ3ewWNFyLoMOzET_e4RwlQNchNByi7wbcMAY7KAoOa7B0CZkbvyuxnX-2oVaYQheMXgYvJDHLAfFZtIhPnfnY7PGd1XaSzmGcVDmEBbnc524_ZAsnzaZCWWo9Bi4_RXXMfgkti2JYRGk1Buw%3D%3D

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=138228&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Kayumov Ayrat Rashitovich	ru_RU
dc.contributor.author	Vedyakin Aleksey	ru_RU
dc.contributor.author	Vishnyakov Innokentiy	ru_RU
dc.contributor.author	Sabancev Anton Vladimirovich	ru_RU
dc.contributor.author	Khodorkovskiy Mikhail Alekseevich	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Vedyaykin A.D. Recombinant FtsZ Proteins from Mollicutes Interactwith Escherichia coli Division Machinery / A.D. Vedyaykin, A.V. Sabantsev, M.A. Khodorkovskii, A.R. Kayumov, I.E. Vishnyakov // BioNanoScience. - 2016. DOI: 10.1007/s12668-016-0248-3.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=138228&p_lang=2	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	FtsZ is a well-known prokaryotic tubulin homo-logue. It participates in cytokinesis in most bacteria and isconsidered to be a key division protein. However, the ge-nomes of some mycoplasma species (class Mollicutes) lackof the ftsZ genes or even ftsZ-like genes. Moreover, it wasdemonstrated for Myco plasma mycoides and Mycoplasmagenitalium that FtsZ protein is not essential for division andsurvival. In this study, we induced an expression of recombi-nant FtsZ proteins from Mollicutes Acholeplasma laidlawiiand Mycoplasma gallisepticum in Escherichia coli cells inthe background of normal expression of its own FtsZ. Byusing indirect doub le immunofluo rescence in combinationwith single-molecule localization microscopy, it was demon-strated that both mycoplasmal FtsZ proteins are able to inter-fere with E. coli division machinery, but the interaction differsdepending on the protein used. Our data suggest that FtsZproteins from Mollicutes may play an important role in thecell division of co	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Cell division	ru_RU
dc.subject	FtsZ	ru_RU
dc.subject	Mycoplasma	ru_RU
dc.subject	Escherichia coli	ru_RU
dc.title	Recombinant FtsZ Proteins from Mollicutes Interactwith Escherichia coli Division Machinery	ru_RU
dc.type	Articles in international journals and collections	ru_RU