KFU. Card publication. Ammonia Attenuates LPS-Induced Upregulation of Pro-Inflammatory Cytokine mRNA in Co-Cultured Astrocytes and Microglia

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

AMMONIA ATTENUATES LPS-INDUCED UPREGULATION OF PRO-INFLAMMATORY CYTOKINE MRNA IN CO-CULTURED ASTROCYTES AND MICROGLIA

Form of presentation

Articles in international journals and collections

Year of publication

2016

��

��

Authors, employees of KFU

Shafigullina Aygul Kasyymovna, author

Other authors

Albrecht Ute , author

Groos-Sahr Katerina , author

Görg Boris , author

Häussinger Dieter , author

Karababa Ayse , author

Keitel Verena , author

Bibliographic description in the original language

Karababa, A. Ammonia Attenuates LPS-Induced Upregulation of Pro-Inflammatory Cytokine mRNA in Co-Cultured Astrocytes and Microglia / A. Karababa, K. Groos-Sahr, U. Albrecht, V. Keitel, A. Shafigullina, B. Görg, D. Häussinger // Neurochem Res. - 2016. - R. 1–13.

Annotation

Hepatic encephalopathy (HE) is associated with cerebral microglia activation. Ammonia, a major toxin of HE, activates microglia in vitro but does not trigger pro-inflammatory cytokine synthesis. In the present study we analysed effects of ammonia on lipopolysaccharide (LPS)-induced upregulation of microglia activation and cytokine mRNA as well as on cytokine secretion in mono-cultured microglia and co-cultured astrocytes and microglia. In mono-cultured microglia LPS (100 ng/ml, 18 h) strongly elevated mRNA levels of the microglia activation marker CD14 and the pro-inflammatory cytokines IL-1α/β, IL-6 and TNF-α. NH4Cl (5 mmol/l) had no effect on LPS-induced upregulation of CD14, IL-1α/β and IL-6 mRNA but enhanced LPS-induced upregulation of TNF-α mRNA in mono-cultured microglia. In co-cultured astrocytes and microglia, however, LPS-induced upregulation of IL-1α/β, TNF-α, IL-6, CD14 but not of IL-10, IL-12A/B or TGFβ1-3 mRNA was attenuated by NH4Cl. LPS-induced upregulation of IL-1α/β, I

Keywords

Ammonia; Hepatic encephalopathy; Neuroinflammation; Neurosteroids; TGR5

The name of the journal

Neurochem Res.

On-line resource for training course

http://dspace.kpfu.ru/xmlui/bitstream/handle/net/108584/Ammonia_Attenuates_LPS_Induced_Karababa_2016.pdf?sequence=1&isAllowed=y

URL

https://www.ncbi.nlm.nih.gov/pubmed/?term=Ammonia+Attenuates+LPS-Induced+Upregulation+of+Pro-Inflammatory+Cytokine+mRNA+in+Co-Cultured+Astrocytes+and+Microglia

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=148271&p_lang=2

Resource files

File name	Size (MB)	Format
Ammonia_Attenuates_LPS_Induced_Karababa_2016.pdf	0,08	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Shafigullina Aygul Kasyymovna	ru_RU
dc.contributor.author	Albrecht Ute	ru_RU
dc.contributor.author	Groos-Sahr Katerina	ru_RU
dc.contributor.author	Görg Boris	ru_RU
dc.contributor.author	Häussinger Dieter	ru_RU
dc.contributor.author	Karababa Ayse	ru_RU
dc.contributor.author	Keitel Verena	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Karababa, A. Ammonia Attenuates LPS-Induced Upregulation of Pro-Inflammatory Cytokine mRNA in Co-Cultured Astrocytes and Microglia / A. Karababa, K. Groos-Sahr, U. Albrecht, V. Keitel, A. Shafigullina, B. Görg, D. Häussinger // Neurochem Res. - 2016. - �. 1–13.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=148271&p_lang=2	ru_RU
dc.description.abstract	Neurochem Res.	ru_RU
dc.description.abstract	Hepatic encephalopathy (HE) is associated with cerebral microglia activation. Ammonia, a major toxin of HE, activates microglia in vitro but does not trigger pro-inflammatory cytokine synthesis. In the present study we analysed effects of ammonia on lipopolysaccharide (LPS)-induced upregulation of microglia activation and cytokine mRNA as well as on cytokine secretion in mono-cultured microglia and co-cultured astrocytes and microglia. In mono-cultured microglia LPS (100 ng/ml, 18 h) strongly elevated mRNA levels of the microglia activation marker CD14 and the pro-inflammatory cytokines IL-1α/β, IL-6 and TNF-α. NH4Cl (5 mmol/l) had no effect on LPS-induced upregulation of CD14, IL-1α/β and IL-6 mRNA but enhanced LPS-induced upregulation of TNF-α mRNA in mono-cultured microglia. In co-cultured astrocytes and microglia, however, LPS-induced upregulation of IL-1α/β, TNF-α, IL-6, CD14 but not of IL-10, IL-12A/B or TGFβ1-3 mRNA was attenuated by NH4Cl. LPS-induced upregulation of IL-1α/β, I	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Ammonia Attenuates LPS-Induced Upregulation of Pro-Inflammatory Cytokine mRNA in Co-Cultured Astrocytes and Microglia	ru_RU
dc.type	Articles in international journals and collections	ru_RU