KFU. Card publication. Targeting microbial biofilms using Ficin, a nonspecific plant protease

KAZAN
FEDERAL UNIVERSITY

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TARGETING MICROBIAL BIOFILMS USING FICIN, A NONSPECIFIC PLANT PROTEASE

Form of presentation

Articles in international journals and collections

Year of publication

2017

Язык

английский

Authors, employees of KFU

Akhatova Farida Serikovna, author

Baydamshina Diana Rafisovna, author

Bogachev Mikhail Igorevich, author

Kayumov Ayrat Rashitovich, author

Rozhina Elvira Vyacheslavovna, author

Trizna Elena Yurevna, author

Fakhrullin Ravil Faridovich, author

Other authors

Artyukhov V. G., author

Holyavka M. G., author

Bibliographic description in the original language

Baidamshina D.R. Targeting microbial biofilms using Ficin, a nonspecific plant protease / D.R. Baidamshina, E.Y. Trizna, M.G. Holyavka, M.I. Bogachev, V.G. Artyukhov, F.S. Akhatova, E.V. Rozhina, R.F. Fakhrullin, A.R. Kayumov // Scientific Reports. - 2017. - V.7. - Art. № 46068.

Annotation

Biofilms, the communities of surface-attached bacteria embedded into extracellular matrix, are ubiquitous microbial consortia securing the effective resistance of constituent cells to environmental impacts and host immune responses. Biofilm-embedded bacteria are generally inaccessible for antimicrobials, therefore the disruption of biofilm matrix is the potent approach to eradicate microbial biofilms. We demonstrate here the destruction of Staphylococcus aureus and Staphylococcus epidermidis biofilms with Ficin, a nonspecific plant protease. The biofilm thickness decreased two-fold after 24 hours treatment with Ficin at 10 μg/ml and six-fold at 1000 μg/ml concentration. We confirmed the successful destruction of biofilm structures and the significant decrease of non-specific bacterial adhesion to the surfaces after Ficin treatment using confocal laser scanning and atomic force microscopy. Importantly, Ficin treatment enhanced the effects of antibiotics on biofilms-embedded cells via di

Keywords

microbial biofilms, AFM, confocal microscopy, antibacterial, ficin

The name of the journal

SCIENTIFIC REPORTS

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017260158&doi=10.1038%2fsrep46068&partnerID=40&md5=6d76c9287d36df3d056c487505a87d33

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=156993&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Akhatova Farida Serikovna	ru_RU
dc.contributor.author	Baydamshina Diana Rafisovna	ru_RU
dc.contributor.author	Bogachev Mikhail Igorevich	ru_RU
dc.contributor.author	Kayumov Ayrat Rashitovich	ru_RU
dc.contributor.author	Rozhina Elvira Vyacheslavovna	ru_RU
dc.contributor.author	Trizna Elena Yurevna	ru_RU
dc.contributor.author	Fakhrullin Ravil Faridovich	ru_RU
dc.contributor.author	Artyukhov V. G.	ru_RU
dc.contributor.author	Holyavka M. G.	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Baidamshina D.R. Targeting microbial biofilms using Ficin, a nonspecific plant protease / D.R. Baidamshina, E.Y. Trizna, M.G. Holyavka, M.I. Bogachev, V.G. Artyukhov, F.S. Akhatova, E.V. Rozhina, R.F. Fakhrullin, A.R. Kayumov // Scientific Reports. - 2017. - V.7. - Art. № 46068.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=156993&p_lang=2	ru_RU
dc.description.abstract	SCIENTIFIC REPORTS	ru_RU
dc.description.abstract	Biofilms, the communities of surface-attached bacteria embedded into extracellular matrix, are ubiquitous microbial consortia securing the effective resistance of constituent cells to environmental impacts and host immune responses. Biofilm-embedded bacteria are generally inaccessible for antimicrobials, therefore the disruption of biofilm matrix is the potent approach to eradicate microbial biofilms. We demonstrate here the destruction of Staphylococcus aureus and Staphylococcus epidermidis biofilms with Ficin, a nonspecific plant protease. The biofilm thickness decreased two-fold after 24 hours treatment with Ficin at 10 μg/ml and six-fold at 1000 μg/ml concentration. We confirmed the successful destruction of biofilm structures and the significant decrease of non-specific bacterial adhesion to the surfaces after Ficin treatment using confocal laser scanning and atomic force microscopy. Importantly, Ficin treatment enhanced the effects of antibiotics on biofilms-embedded cells via di	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	microbial biofilms	ru_RU
dc.subject	AFM	ru_RU
dc.subject	confocal microscopy	ru_RU
dc.subject	antibacterial	ru_RU
dc.subject	ficin	ru_RU
dc.title	Targeting microbial biofilms using Ficin, a nonspecific plant protease	ru_RU
dc.type	Articles in international journals and collections	ru_RU