KFU. Card publication. Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

NON-RADIOACTIVE TRF ASSAY MODIFICATIONS TO IMPROVE TELOMERIC DNA DETECTION EFFICIENCY IN PLANTS

Form of presentation

Articles in international journals and collections

Year of publication

2016

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Authors, employees of KFU

Abdulkina Liliya Rinatovna, author

Bibliographic description in the original language

Nigmatullina, L.R. Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants / L.R. Nigmatullina, M.R. Sharipova, E.V. Shakirov // BioNanoScience.- 2016.- V. 6.- I. 4.-P. 325–328.

Annotation

The length of telomeric DNA is often considered a cellular biomarker of aging and general health status. Several telomere length measuring assays have been developed, of which the most common is the Telomere Restriction Fragment (TRF) analysis, which typically involves the use of radioactively labeled oligonucleotide probes. While highly effective, this method potentially poses substantial health concerns and generates radioactive waste. Digoxigenin (DIG) alternatives to radioactive probes have been developed and used successfully in a number of assays. Here we optimize the DIG protocol to measure telomere length in the model plant Arabidopsis thaliana and present evidence that this approach can be used successfully to efficiently and accurately measure telomere length in plants. Specifically, hybridization temperature of 42 C instead of the typical 55 C appears to generate stronger signals. In addition, DIG incorporation at 5'-end instead of 3'-end of the labeled oligonucleotide great

Keywords

Nonradioactive telomere restriction fragment analysis, Southern blot, DNA, Aging, Plant

The name of the journal

BioNanoScience

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=160114&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Abdulkina Liliya Rinatovna	ru_RU
dc.date.accessioned	2016-01-01T00:00:00Z	ru_RU
dc.date.available	2016-01-01T00:00:00Z	ru_RU
dc.date.issued	2016	ru_RU
dc.identifier.citation	Nigmatullina, L.R. Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants / L.R. Nigmatullina, M.R. Sharipova, E.V. Shakirov // BioNanoScience.- 2016.- V. 6.- I. 4.-P. 325–328.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=160114&p_lang=2	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	The length of telomeric DNA is often considered a cellular biomarker of aging and general health status. Several telomere length measuring assays have been developed, of which the most common is the Telomere Restriction Fragment (TRF) analysis, which typically involves the use of radioactively labeled oligonucleotide probes. While highly effective, this method potentially poses substantial health concerns and generates radioactive waste. Digoxigenin (DIG) alternatives to radioactive probes have been developed and used successfully in a number of assays. Here we optimize the DIG protocol to measure telomere length in the model plant Arabidopsis thaliana and present evidence that this approach can be used successfully to efficiently and accurately measure telomere length in plants. Specifically, hybridization temperature of 42 C instead of the typical 55 C appears to generate stronger signals. In addition, DIG incorporation at 5'-end instead of 3'-end of the labeled oligonucleotide great	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Nonradioactive telomere restriction fragment analysis	ru_RU
dc.subject	Southern blot	ru_RU
dc.subject	DNA	ru_RU
dc.subject	Aging	ru_RU
dc.subject	Plant	ru_RU
dc.title	Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants	ru_RU
dc.type	Articles in international journals and collections	ru_RU