KFU. Card publication. Extraction and serological properties of Mycobacterium cell surface and excreted proteins

KAZAN
FEDERAL UNIVERSITY

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- 中文
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EXTRACTION AND SEROLOGICAL PROPERTIES OF MYCOBACTERIUM CELL SURFACE AND EXCRETED PROTEINS

Form of presentation

Articles in international journals and collections

Year of publication

2018

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Authors, employees of KFU

Aleksandrova Natalya Mikhaylovna, author

Valeeva Anna Rafkatovna, author

Efimova Marina Anatolevna, author

Mukminov Malik Nilovich, author

Nabatov Aleksey Anatolevich, author

Shuralev Eduard Arkadevich, author

Other authors

Akhmadeev R. M., author

Ivanov A. V., author

Khaertynov K. S., author

Khaertynova I. M., author

Moskvicheva A. V., author

Urazov N. G., author

Authors, postgraduates KFU

Samigullina Elvira Shamilevna, postgraduate kfu

Bibliographic description in the original language

Khaertynov, K.S., Valeeva, A.R., Ivanov, A.V., Mukminov, M.N., Urazov, N.G., Khaertynova, I.M., Aleksandrova, N.M., Moskvicheva, A.V., Efimova, M.A., Akhmadeev, R.M., Samigullina, E.S., Nabatov, A.A., Shuralev, E.A. Extraction and serological properties of Mycobacterium cell surface and excreted proteins // BioNanoScience. - 2018. - Vol.8, Is.1. - P.459-466. doi.org/10.1007/s12668-017-0492-1

Annotation

Here, we extracted Mycobacterium tuberculosis H37Rv proteins from culture medium or from the cell surface and studied their reactivity with anti-M. tuberculosis serum in both ELISA and immunoblots. We found that M. tuberculosis surface proteins, extracted using dimethyl sulfoxide, with molecular weights in the range of 6.5–200 kDa, showed strong specific reactivity with anti-M. tuberculosis positive serum. While excreted proteins in the molecular weight range of 32–45 kDa had the highest reactivity.

Keywords

Mycobacterium tuberculosis, proteins, DMSO, antigens, Vivaspin, immunoblot

The name of the journal

BioNanoScience

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044383044&doi=10.1007%2fs12668-017-0492-1&partnerID=40&md5=89ff297d1d5590741e0bee33f080e9f1

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=179439&p_lang=2

Resource files

File name	Size (MB)	Format
F_Khaertynov_Extraction_BNS_2018.pdf	1,96	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Aleksandrova Natalya Mikhaylovna	ru_RU
dc.contributor.author	Valeeva Anna Rafkatovna	ru_RU
dc.contributor.author	Efimova Marina Anatolevna	ru_RU
dc.contributor.author	Mukminov Malik Nilovich	ru_RU
dc.contributor.author	Nabatov Aleksey Anatolevich	ru_RU
dc.contributor.author	Shuralev Eduard Arkadevich	ru_RU
dc.contributor.author	Akhmadeev R. M.	ru_RU
dc.contributor.author	Ivanov A. V.	ru_RU
dc.contributor.author	Khaertynov K. S.	ru_RU
dc.contributor.author	Khaertynova I. M.	ru_RU
dc.contributor.author	Moskvicheva A. V.	ru_RU
dc.contributor.author	Urazov N. G.	ru_RU
dc.contributor.author	Samigullina Elvira Shamilevna	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	Khaertynov, K.S., Valeeva, A.R., Ivanov, A.V., Mukminov, M.N., Urazov, N.G., Khaertynova, I.M., Aleksandrova, N.M., Moskvicheva, A.V., Efimova, M.A., Akhmadeev, R.M., Samigullina, E.S., Nabatov, A.A., Shuralev, E.A. Extraction and serological properties of Mycobacterium cell surface and excreted proteins // BioNanoScience. - 2018. - Vol.8, Is.1. - P.459-466. doi.org/10.1007/s12668-017-0492-1	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=179439&p_lang=2	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	Here, we extracted Mycobacterium tuberculosis H37Rv proteins from culture medium or from the cell surface and studied their reactivity with anti-M. tuberculosis serum in both ELISA and immunoblots. We found that M. tuberculosis surface proteins, extracted using dimethyl sulfoxide, with molecular weights in the range of 6.5–200 kDa, showed strong specific reactivity with anti-M. tuberculosis positive serum. While excreted proteins in the molecular weight range of 32–45 kDa had the highest reactivity.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Mycobacterium tuberculosis	ru_RU
dc.subject	proteins	ru_RU
dc.subject	DMSO	ru_RU
dc.subject	antigens	ru_RU
dc.subject	Vivaspin	ru_RU
dc.subject	immunoblot	ru_RU
dc.title	Extraction and serological properties of Mycobacterium cell surface and excreted proteins	ru_RU
dc.type	Articles in international journals and collections	ru_RU