KFU. Card publication. Investigating nano particles in bioinformatic analysis of the brucella genome for indication and differentation by qPCR

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

INVESTIGATING NANO PARTICLES IN BIOINFORMATIC ANALYSIS OF THE BRUCELLA GENOME FOR INDICATION AND DIFFERENTATION BY QPCR

Form of presentation

Articles in international journals and collections

Year of publication

2018

��

��

Authors, employees of KFU

Shuralev Eduard Arkadevich, author

Other authors

Kosarev M. A., author

Osyanin K. A., author

Khammadov N. I., author

Authors, students KFU

Khammadova Alfiya Vasilevna, author

Bibliographic description in the original language

Khammadova A.V., Khammadov N.I., Kosarev M.A., Osyanin K.A., Shuralev E.A. Investigating nano particles in bioinformatic analysis of the brucella genome for indication and differentation by qPCR // International Journal of Engineering and Technology(UAE). - 2018. - Vol.7, Is.4.7 Special Issue 7. - P.227-230.

Annotation

Based on the identified genes, the design of specific primers and probes for qPCR was made, which makes it possible to differentiate B. melitensis and B. abortus in a single reaction. For the differentiation of Brucella strains of the same species, the PCR method of multilocus VNTR analysis, optimized for uniform temperature conditions, is described. In addition, MLVA profiles for B. abortus and B. melitensis genomes are presented. All methods of indication and differentiation of Brucella species and strains are described in detail, which allows them to be used in the everyday work of any PCR laboratory dealing with brucellosis. Control of amplification in the indication of bacteria of the genus Brucella and determination of their belonging to B. abortus and B. melitensis species is recommended to be carried out using the plasmid DNA designed in this work.

Keywords

Brucella, genome, indication, nano particle, identification, qPCR, MLVA

The name of the journal

International Journal of Engineering and Technology(UAE)

URL

https://www.scopus.com/record/display.uri?eid=2-s2.0-85075105336&origin=inward&txGid=bc752bc06069daf694435e0af8873d17

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=187192&p_lang=2

Resource files

File name	Size (MB)	Format
Khammadova_Brucella.pdf	0,28	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Shuralev Eduard Arkadevich	ru_RU
dc.contributor.author	Kosarev M. A.	ru_RU
dc.contributor.author	Osyanin K. A.	ru_RU
dc.contributor.author	Khammadov N. I.	ru_RU
dc.contributor.author	Khammadova Alfiya Vasilevna	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	Khammadova A.V., Khammadov N.I., Kosarev M.A., Osyanin K.A., Shuralev E.A. Investigating nano particles in bioinformatic analysis of the brucella genome for indication and differentation by qPCR // International Journal of Engineering and Technology(UAE). - 2018. - Vol.7, Is.4.7 Special Issue 7. - P.227-230.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=187192&p_lang=2	ru_RU
dc.description.abstract	International Journal of Engineering and Technology(UAE)	ru_RU
dc.description.abstract	Based on the identified genes, the design of specific primers and probes for qPCR was made, which makes it possible to differentiate B. melitensis and B. abortus in a single reaction. For the differentiation of Brucella strains of the same species, the PCR method of multilocus VNTR analysis, optimized for uniform temperature conditions, is described. In addition, MLVA profiles for B. abortus and B. melitensis genomes are presented. All methods of indication and differentiation of Brucella species and strains are described in detail, which allows them to be used in the everyday work of any PCR laboratory dealing with brucellosis. Control of amplification in the indication of bacteria of the genus Brucella and determination of their belonging to B. abortus and B. melitensis species is recommended to be carried out using the plasmid DNA designed in this work.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Brucella	ru_RU
dc.subject	genome	ru_RU
dc.subject	indication	ru_RU
dc.subject	nano particle	ru_RU
dc.subject	identification	ru_RU
dc.subject	qPCR	ru_RU
dc.subject	MLVA	ru_RU
dc.title	Investigating nano particles in bioinformatic analysis of the brucella genome for indication and differentation by qPCR	ru_RU
dc.type	Articles in international journals and collections	ru_RU