KFU. Card publication. Influence of long-term cultivation and cryopreservation on phenotype of rat hepatic stellate cells

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FEDERAL UNIVERSITY

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INFLUENCE OF LONG-TERM CULTIVATION AND CRYOPRESERVATION ON PHENOTYPE OF RAT HEPATIC STELLATE CELLS

Form of presentation

Conference proceedings in international journals and collections

Year of publication

2018

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Authors, employees of KFU

Gumerova Anisa Azatovna, author

Kiyasov Andrey Pavlovich, author

Rizvanov Albert Anatolevich, author

Titova Angelina Andreevna, author

Titova Marina Aleksandrovna, author

Shafigullina Aygul Kasyymovna, author

Authors, students KFU

Garipov Ilnur Ilgizovich, author

Bibliographic description in the original language

Garipov I.I. Influence of long-term cultivation and cryopreservation on phenotype of rat hepatic stellate cells / I.I. Garipov, A.K. Shafigullina, A.A. Titova, M.A. Titova, A.A. Rizvanov, A.A. Gumerova, A.P. Kiyasov // Annual Congress of European Society of Gene and Cell Therapy, November 2018, Lausanne, Switzerland. - HUMAN GENE THERAPY 27: P. A134-135.

Annotation

HUMAN GENE THERAPY 27 (2018)

Keywords

hepatic stellate cells, cryopreservation, phenotype, long-term cultivation

The name of the journal

HUMAN GENE THERAPY 27 (2018)

On-line resource for training course

http://dspace.kpfu.ru/xmlui/bitstream/handle/net/146668/ESGCT_1_Garipov_I.pdf?sequence=1&isAllowed=y

URL

https://www.esgct.eu/Congress/Current-Congress.aspx

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=188567&p_lang=2

Resource files

File name	Size (MB)	Format
ESGCT_1_Garipov_I.pdf	0,07	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Gumerova Anisa Azatovna	ru_RU
dc.contributor.author	Kiyasov Andrey Pavlovich	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Titova Angelina Andreevna	ru_RU
dc.contributor.author	Titova Marina Aleksandrovna	ru_RU
dc.contributor.author	Shafigullina Aygul Kasyymovna	ru_RU
dc.contributor.author	Garipov Ilnur Ilgizovich	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	Garipov I.I. Influence of long-term cultivation and cryopreservation on phenotype of rat hepatic stellate cells / I.I. Garipov, A.K. Shafigullina, A.A. Titova, M.A. Titova, A.A. Rizvanov, A.A. Gumerova, A.P. Kiyasov // Annual Congress of European Society of Gene and Cell Therapy, November 2018, Lausanne, Switzerland. - HUMAN GENE THERAPY 27: P. A134-135.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=188567&p_lang=2	ru_RU
dc.description.abstract	HUMAN GENE THERAPY 27 (2018)	ru_RU
dc.description.abstract	Many organs and tissues have been reported to harbor regional stem cells. One of the candidates for this role in the liver is hepatic stellate cells (HSC). Cultivation of cells in vitro may lead to changes in their morphology and phenotype. The risk of changes increases with long-term cultivation (passage 5 and above), as well as with cryopreservation of cells. Freezing allows storage of isolated cell; however, the method can influence cells phenotype and properties. The aim of research was to study phenotype of rat HSC during long-term cultivation and cryopreservation. Three cultures of HSC (I ? after cryopreservation, II ? long-term culture, passage 6, and III ? control, passage 4) were stained immunocytochemically with antibodies to desmin, α-SMA, CK19, Ki-67; quantitative real-time PCR analysis was also performed to evaluate desmin and α-SMA gene expression. It was observed that morphology of cells was similar in all three groups. In groups with long-term cultivation and cryopreser	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	hepatic stellate cells	ru_RU
dc.subject	cryopreservation	ru_RU
dc.subject	phenotype	ru_RU
dc.subject	long-term cultivation	ru_RU
dc.title	Influence of long-term cultivation and cryopreservation on phenotype of rat hepatic stellate cells	ru_RU
dc.type	Conference proceedings in international journals and collections	ru_RU