KFU. Card publication. In-vitro antitumor activity of new quaternary phosphonium salts, derivatives of 3-hydroxypyridine.

KAZAN
FEDERAL UNIVERSITY

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IN-VITRO ANTITUMOR ACTIVITY OF NEW QUATERNARY PHOSPHONIUM SALTS, DERIVATIVES OF 3-HYDROXYPYRIDINE.

Form of presentation

International monographs

Year of publication

2018

Authors, employees of KFU

Akhunzyanov Almaz Anasovich, author

Gabbasova Railya Raushanovna, author

Iksanova Alfiya Gabdulakhatovna, author

Authors, students KFU

Kupriyanova Tatyana Viktorovna, author

Bibliographic description in the original language

This work presents the results of in-vitro biological activity studies of three novel anticancer agents, phosphonium salts based on the 3-hydroxypyridine scaffold, including one derivative of 4-deoxypyridoxine. Proliferation and viability of cells treated with these compounds was assessed by the colony formation and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Effects of the compounds on apoptosis and cell cycle were studied by flow cytometry using annexin V-FITC/propidium iodide and propidium iodide staining, respectively. The influence of the compounds on mitochondrial membrane potential and intracellular reactive oxygen species was evaluated using tetramethyl rhodamine ethyl and DCFHA staining. Western blot analysis was used to study the changes in the expression of Bcl-xL, Bax, and caspase-3 apoptotic proteins. The treatment of ovarian adenocarcinoma cells OVCAR-4 with the tested compounds inhibited the growth and induced cell cycle arrest in the G1 phase. 3

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=192257&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Akhunzyanov Almaz Anasovich	ru_RU
dc.contributor.author	Gabbasova Railya Raushanovna	ru_RU
dc.contributor.author	Iksanova Alfiya Gabdulakhatovna	ru_RU
dc.contributor.author	Kupriyanova Tatyana Viktorovna	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	This work presents the results of in-vitro biological activity studies of three novel anticancer agents, phosphonium salts based on the 3-hydroxypyridine scaffold, including one derivative of 4-deoxypyridoxine. Proliferation and viability of cells treated with these compounds was assessed by the colony formation and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Effects of the compounds on apoptosis and cell cycle were studied by flow cytometry using annexin V-FITC/propidium iodide and propidium iodide staining, respectively. The influence of the compounds on mitochondrial membrane potential and intracellular reactive oxygen species was evaluated using tetramethyl rhodamine ethyl and DCFHA staining. Western blot analysis was used to study the changes in the expression of Bcl-xL, Bax, and caspase-3 apoptotic proteins. The treatment of ovarian adenocarcinoma cells OVCAR-4 with the tested compounds inhibited the growth and induced cell cycle arrest in the G1 phase. 3	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=192257&p_lang=2	ru_RU
dc.language.iso	ru	ru_RU
dc.title	In-vitro antitumor activity of new quaternary phosphonium salts, derivatives of 3-hydroxypyridine.	ru_RU
dc.type	International monographs	ru_RU