KFU. Card publication. Biochemical characterization of peroxidases from the moss Dicranum scoparium

KAZAN
FEDERAL UNIVERSITY

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BIOCHEMICAL CHARACTERIZATION OF PEROXIDASES FROM THE MOSS DICRANUM SCOPARIUM

Form of presentation

Articles in international journals and collections

Year of publication

2018

Язык

английский

Authors, employees of KFU

Minibaeva Farida Vilevna, author

Chasov Andrey Vasilevich, author

Other authors

Beckett Peter Beckett, author

Viktorova Larisa Viktorovna, author

Trifonova Tatyana Vladimirovna, author

Authors, postgraduates KFU

Onele Alfred Obinna , postgraduate kfu

Bibliographic description in the original language

Onele A.O. Biochemical characterization of peroxidases from the moss Dicranum scoparium / A.O. Onele, A. Chasov, L. Viktorova, R.P. Beckett, T. Trifonova, F. Minibayeva // South African Journal of Botany. - 2018. - V. 119. - P. 132-141.

Annotation

Mosses are a convenient model to study stress responses of plants because of their remarkable stress tolerance. Peroxidase (EC 1.11.1.7) activities were tested in three moss species, namely Dicranum scoparium, Hylocomium splendens and Pleurozium schreberi growing together in the same location in a boreal forest. Peroxidase activity in D. scoparium was twice as high as in other mosses. Total peroxidase activity in unstressed D. scoparium was constitutively high; furthermore, long-term desiccation caused a significant increase in activity after 48 h of drying. Interestingly, when thalli desiccated for a week were rapidly rehydrated, peroxidase activity initially declined and then increased after 2 h rehydration. Diverse anionic and cationic isoforms were detected by native isoelectric focusing and PAGE of both crude extracts and partially purified peroxidases. The ability of peroxidases from D. scoparium to produce superoxide radical (O2 ?−) was confirmed using the 2,3-bis-(2-methoxy-4-nitro-5- sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay and in-gel nitroblue tetrazolium chloride (NBT) staining; specific O2 ?− producing isoforms were revealed using 2D electrophoresis. Given a quinone and chelated Fe3+D. scoparium could produce extracellular hydroxyl radical (?OH), and production was increased by desiccation/rehydration stress. The possible roles of peroxidases and quinone reductases in apoplastic ?OH production is discussed. Our data demonstrate that D. scoparium possesses high constitutive peroxidase activity that can be further increased by desiccation stress. Among the diverse moss peroxidases, some anionic isoforms displayed both pro- and antioxidative activities. These findings suggest that the ability of peroxidases to produce and detoxify reactive oxygen species is an evolutionarily ancient characteristic, important for plant stress tolerance.

Keywords

Dicranum scoparium Hedw., Bryophytes, Mosses, Desiccation tolerance, Peroxidase isoforms, Superoxide radical

The name of the journal

South African Journal of Botany

URL

https://www.sciencedirect.com/science/article/pii/S0254629918310251?via%3Dihub

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=202763&p_lang=2

Resource files

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Biochemical_characterization_of_peroxidases_from_the_moss_Dicranum_scoparium.pdf	1,39	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Minibaeva Farida Vilevna	ru_RU
dc.contributor.author	Chasov Andrey Vasilevich	ru_RU
dc.contributor.author	Beckett Peter Beckett	ru_RU
dc.contributor.author	Viktorova Larisa Viktorovna	ru_RU
dc.contributor.author	Trifonova Tatyana Vladimirovna	ru_RU
dc.contributor.author	Onele Alfred Obinna	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	Onele A.O. Biochemical characterization of peroxidases from the moss Dicranum scoparium / A.O. Onele, A. Chasov, L. Viktorova, R.P. Beckett, T. Trifonova, F. Minibayeva // South African Journal of Botany. - 2018. - V. 119. - P. 132-141.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=202763&p_lang=2	ru_RU
dc.description.abstract	South African Journal of Botany	ru_RU
dc.description.abstract	Mosses are a convenient model to study stress responses of plants because of their remarkable stress tolerance. Peroxidase (EC 1.11.1.7) activities were tested in three moss species, namely Dicranum scoparium, Hylocomium splendens and Pleurozium schreberi growing together in the same location in a boreal forest. Peroxidase activity in D. scoparium was twice as high as in other mosses. Total peroxidase activity in unstressed D. scoparium was constitutively high; furthermore, long-term desiccation caused a significant increase in activity after 48 h of drying. Interestingly, when thalli desiccated for a week were rapidly rehydrated, peroxidase activity initially declined and then increased after 2 h rehydration. Diverse anionic and cationic isoforms were detected by native isoelectric focusing and PAGE of both crude extracts and partially purified peroxidases. The ability of peroxidases from D. scoparium to produce superoxide radical (O2 ?−) was confirmed using the 2,3-bis-(2-methoxy-4-nitro-5- sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay and in-gel nitroblue tetrazolium chloride (NBT) staining; specific O2 ?− producing isoforms were revealed using 2D electrophoresis. Given a quinone and chelated Fe3+D. scoparium could produce extracellular hydroxyl radical (?OH), and production was increased by desiccation/rehydration stress. The possible roles of peroxidases and quinone reductases in apoplastic ?OH production is discussed. Our data demonstrate that D. scoparium possesses high constitutive peroxidase activity that can be further increased by desiccation stress. Among the diverse moss peroxidases, some anionic isoforms displayed both pro- and antioxidative activities. These findings suggest that the ability of peroxidases to produce and detoxify reactive oxygen species is an evolutionarily ancient characteristic, important for plant stress tolerance.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Dicranum scoparium Hedw.	ru_RU
dc.subject	Bryophytes	ru_RU
dc.subject	Mosses	ru_RU
dc.subject	Desiccation tolerance	ru_RU
dc.subject	Peroxidase isoforms	ru_RU
dc.subject	Superoxide radical	ru_RU
dc.title	Biochemical characterization of peroxidases from the moss Dicranum scoparium	ru_RU
dc.type	Articles in international journals and collections	ru_RU