KFU. Card publication. Reaction of liver cells to streptozocin-nicotinamide- induced diabetes mellitus in mice

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FEDERAL UNIVERSITY

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REACTION OF LIVER CELLS TO STREPTOZOCIN-NICOTINAMIDE- INDUCED DIABETES MELLITUS IN MICE

Form of presentation

Conference proceedings in international journals and collections

Year of publication

2019

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Authors, employees of KFU

Kaligin Maksim Sergeevich, author

Kiyasov Andrey Pavlovich, author

Mavlikeev Mikhail Olegovich, author

Rizvanov Albert Anatolevich, author

Titova Angelina Andreevna, author

Chernova Olga Nikolaevna, author

Shafigullina Aygul Kasyymovna, author

Yakovleva Olga Vladislavovna, author

Bibliographic description in the original language

Shafigullina A.K. Reaction of liver cells to streptozocin-nicotinamide-induced diabetes mellitus in mice / A.K. Shafigullina, M.S. Kaligin, M.O. Mavlikeev, O.N. Chernova, O.V. Yakovleva, A.A. Titova, A.A. Rizvanov, A.P. Kiyasov // Annual Congress of European Society of Gene and Cell Therapy, 22-25 October 2019, Barcelona, Spain. - HUMAN GENE THERAPY: (P470) – P.156-157.

Annotation

HUMAN GENE THERAPY

Keywords

streptozocin-nicotinamide, diabetes mellitus, liver, hepatic stellate cells, myofibroblasts, macrophages

The name of the journal

HUMAN GENE THERAPY

On-line resource for training course

http://dspace.kpfu.ru/xmlui/bitstream/handle/net/151976/F_ESGCT_Aygul_2019_250_words.pdf?sequence=1&isAllowed=y

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=213549&p_lang=2

Resource files

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F_ESGCT_Aygul_2019_250_words.pdf	0,15	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Kaligin Maksim Sergeevich	ru_RU
dc.contributor.author	Kiyasov Andrey Pavlovich	ru_RU
dc.contributor.author	Mavlikeev Mikhail Olegovich	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Titova Angelina Andreevna	ru_RU
dc.contributor.author	Chernova Olga Nikolaevna	ru_RU
dc.contributor.author	Shafigullina Aygul Kasyymovna	ru_RU
dc.contributor.author	Yakovleva Olga Vladislavovna	ru_RU
dc.date.accessioned	2019-01-01T00:00:00Z	ru_RU
dc.date.available	2019-01-01T00:00:00Z	ru_RU
dc.date.issued	2019	ru_RU
dc.identifier.citation	Shafigullina A.K. Reaction of liver cells to streptozocin-nicotinamide-induced diabetes mellitus in mice / A.K. Shafigullina, M.S. Kaligin, M.O. Mavlikeev, O.N. Chernova, O.V. Yakovleva, A.A. Titova, A.A. Rizvanov, A.P. Kiyasov // Annual Congress of European Society of Gene and Cell Therapy, 22-25 October 2019, Barcelona, Spain. - HUMAN GENE THERAPY: (P470) – P.156-157.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=213549&p_lang=2	ru_RU
dc.description.abstract	HUMAN GENE THERAPY	ru_RU
dc.description.abstract	One of diabetes mellitus (DM) animal models is performed by injection of streptozocin-nicotinamide (SZC-NA) – agents that via GLUT-2 transporter blockage affect glucose transport into cells. GLUT-2 is highly present on pancreatic β-cells that explain toxicity of STZ to them and application of SZN-NA to model DM. GLUT-2 is present also on hepatocytes, which participate in maintaining glucose levels and deposit it as glycogen. Damage of hepatocytes is always accompanied by activation of hepatic stellate cells (HSC) that transform into collagen-producing myofibroblasts, resulting in liver fibrosis. The aim of the research was to study reaction of liver cells to SZN-NA-induced DM in mice. Experimental groups: 1) control mice (C57Bl/J); 2) mice with intraperitoneal injection of SZC-NA (100mg/kg). Liver paraffin sections (40 days after) were stained by Mallory's trichrome (connective tissue), PAS (glycogen content), immunohistochemically (IHC) with antibodies to CD163 (macrophages), desmin (HSC), α-SMA (myofibroblasts). Diabetes development was proved by increase of blood sugar and decrease of insulin-secreting β-cells in pancreas. In the liver there was hydropic degeneration of hepatocytes with reduction of glycogen content, mixed polymorphonuclear-mononuclear infiltration including CD163+macrophages. IHC to desmin demonstrated reduced number of desmin+HSC in compare to control group. IHC to α-SMA showed no myofibroblasts. Features of fibrosis were excluded by Mallory's trichrome staining to connective tissue. Thus, SCZ-NA-induced DM is accompanied by damage of glycogen content in hepatocytes, inflammation with Kupffer macrophages activation. The HSC are inhibited, they don`t transform into myofibroblasts, there is no liver fibrosis development. Work supported by Program of Competitive Growth of KFU.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	streptozocin-nicotinamide	ru_RU
dc.subject	diabetes mellitus	ru_RU
dc.subject	liver	ru_RU
dc.subject	hepatic stellate cells	ru_RU
dc.subject	myofibroblasts	ru_RU
dc.subject	macrophages	ru_RU
dc.title	Reaction of liver cells to streptozocin-nicotinamide- induced diabetes mellitus in mice	ru_RU
dc.type	Conference proceedings in international journals and collections	ru_RU