KFU. Card publication. Pleiotropic ef-fect of salt stress on motility and synthesis of secreted ribonucleases by Bacillus pumilus

KAZAN
FEDERAL UNIVERSITY

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PLEIOTROPIC EF-FECT OF SALT STRESS ON MOTILITY AND SYNTHESIS OF SECRETED RIBONUCLEASES BY BACILLUS PUMILUS

Form of presentation

Articles in Russian journals and collections

Year of publication

2017

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Authors, employees of KFU

Evtyugin Vladimir Gennadevich, author

Kolpakov Aleksey Ivanovich, author

Kharitonova Mayya Aleksandrovna, author

Bibliographic description in the original language

Kharitonova M.A. Pleiotropic ef-fect of salt stress on motility and synthesis of secreted ribonucleases by Bacillus pumilus / M.A. Khari-tonova, V.G. Evtugyn, A.I. Kolpakov // BioNanoScience. – 2017. – V.7, I. 4. – P. 623 - 626.

Annotation

Members of the genus Bacillus can successfully counteract a sudden increase in salinity. In addition to the accumulation of osmolytes, saline stress also affects other aspects of bacterial physiology such as exoenzymes synthesis and motility. Here, we have shown that increase of salinity in growth medium leads to elevated biosynthesis level of lowmolecular weight ribonuclease (RNase) binase I from Bacillus pumilus. The same effect was established previously for highmolecular weight binase II. Transmission electron microscopy revealed the absence of flagella and some other changes in salt-stressed cells of B. pumilis. We also detected the gene sequences homologous to the recognition sites of response regulator DegU in the binase I and binase II promoters. Using the B. subtilis strains with various mutations in DegU gene, we found that the two-component signal transduction system DegS-DegU which regulates the motility under salt stress participates in the control of biosynthesis for both secreted RNase of B. pumilis (binase I and binase II).

Keywords

Ribonuclease . Binase I . Binase II . Bacillus pumilus . Salt stress . Motility . Flagella

The name of the journal

BioNanoScience

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=221381&p_lang=2

Resource files

File name	Size (MB)	Format
Kharitonova_et_al_2017_BioNanoScience.pdf	0,49	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Evtyugin Vladimir Gennadevich	ru_RU
dc.contributor.author	Kolpakov Aleksey Ivanovich	ru_RU
dc.contributor.author	Kharitonova Mayya Aleksandrovna	ru_RU
dc.date.accessioned	2017-01-01T00:00:00Z	ru_RU
dc.date.available	2017-01-01T00:00:00Z	ru_RU
dc.date.issued	2017	ru_RU
dc.identifier.citation	Kharitonova M.A. Pleiotropic ef-fect of salt stress on motility and synthesis of secreted ribonucleases by Bacillus pumilus / M.A. Khari-tonova, V.G. Evtugyn, A.I. Kolpakov // BioNanoScience. – 2017. – V.7, I. 4. – P. 623 - 626.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=221381&p_lang=2	ru_RU
dc.description.abstract	BioNanoScience	ru_RU
dc.description.abstract	Members of the genus Bacillus can successfully counteract a sudden increase in salinity. In addition to the accumulation of osmolytes, saline stress also affects other aspects of bacterial physiology such as exoenzymes synthesis and motility. Here, we have shown that increase of salinity in growth medium leads to elevated biosynthesis level of lowmolecular weight ribonuclease (RNase) binase I from Bacillus pumilus. The same effect was established previously for highmolecular weight binase II. Transmission electron microscopy revealed the absence of flagella and some other changes in salt-stressed cells of B. pumilis. We also detected the gene sequences homologous to the recognition sites of response regulator DegU in the binase I and binase II promoters. Using the B. subtilis strains with various mutations in DegU gene, we found that the two-component signal transduction system DegS-DegU which regulates the motility under salt stress participates in the control of biosynthesis for both secreted RNase of B. pumilis (binase I and binase II).	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Pleiotropic ef-fect of salt stress on motility and synthesis of secreted ribonucleases by Bacillus pumilus	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU