KFU. Card publication. Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus

KAZAN
FEDERAL UNIVERSITY

- RUS
- 中文
- ES

INDUCTION OF APOPTOSIS AND AUTOPHAGY IN T-LYMPHOCYTES OF PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS

Form of presentation

Articles in Russian journals and collections

Year of publication

2020

��

��

Authors, employees of KFU

Abramov Sergey Nikolaevich, author

Abramova Zinaida Ivanovna, author

Andrianova Izabella Aleksandrovna, author

Biktagirova Elnara Mauletovna, author

Ibragimov Bulat Rafisovich, author

Skibo Yuliya Valerevna, author

Fatkhullina Aliya Rinatovna, author

Authors, postgraduates KFU

Ibragimov Bulat Rafisovich, postgraduate kfu

Bibliographic description in the original language

Skibo Y.V. Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus/Y.V. Skibo, A.R. Fathullina, B.R.Ibragimov, S.N.Abramov, R.R. Ismagilova, E.M. Biktagirova, I.A. Andrianova1, A.N. Maksudova, Z.I. Abramova // Kazan Medical Journal.- 2020.-V. 101, No. 3.- P.1-8

Annotation

Aim. To analyze the expression of key apoptosis (Bcl-2, caspase-3) and autophagy (Beclin 1, Vps34, p62 and LC3) proteins regulators in peripheral blood T-lymphocytes of patients with systemic lupus erythematosus.Methods. The object of the study was peripheral blood T-lymphocytes of healthy donors and patients with systemic lupus erythematosus. To obtain T cells, we used the immunomagnetic separation method. Protein expression was analyzed using the Western blot method. Statistically analyzing the results was performed using the R software environment. The data was represented using boxplots. Groups were compared using the Mann?Whitney test.Results. According to the results of the study of the apoptotic proteins, we found an increased content of caspase-3 and the absence of significant changes in the content of the anti-apoptotic protein Bcl-2 in patients with lupus, which indicates active apoptotic activity. A comparative analysis of Beclin 1 and Vps34 showed their increased content in the cells of patients, which indicates the activation of autophagy. The analysis of two isoforms of LC3 protein revealed their low content in the group of patients. Since the scatter of indicators was very different from the average value, we analyzed these indicators depending on the severity of the disease. In the acute course group, high content of protein LC3-I was detected, the content of form II was lower. In the group with the subacute course, the number of both isoforms is lower than in the other groups. In the group with a chronic course, significant increases of protein LC3-II and a decrease in the ratio of LC3-I/LC3-II were found.Conclusion. The study showed that depending on the severity of systemic lupus erythematosus, the content of protein LC3 isoforms changes, which can be used for differential diagnosis of disease forms.

Keywords

Bcl-2, caspase-3, Beclin 1, Vps34, LC3, apoptosis, autophagy, systemic lupus erythematosus

The name of the journal

Kazan Medical Journal

URL

https://kazanmedjournal.ru/kazanmedj/article/view/25798/pdf_en

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=234180&p_lang=2

Resource files

File name	Size (MB)	Format
Skibo_KazMedZhurnal25798_62859_1_PB__1_.pdf	0,57	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Abramov Sergey Nikolaevich	ru_RU
dc.contributor.author	Abramova Zinaida Ivanovna	ru_RU
dc.contributor.author	Andrianova Izabella Aleksandrovna	ru_RU
dc.contributor.author	Biktagirova Elnara Mauletovna	ru_RU
dc.contributor.author	Ibragimov Bulat Rafisovich	ru_RU
dc.contributor.author	Skibo Yuliya Valerevna	ru_RU
dc.contributor.author	Fatkhullina Aliya Rinatovna	ru_RU
dc.contributor.author	Ibragimov Bulat Rafisovich	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Skibo Y.V. Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus/Y.V. Skibo, A.R. Fathullina, B.R.Ibragimov, S.N.Abramov, R.R. Ismagilova, E.M. Biktagirova, I.A. Andrianova1, A.N. Maksudova, Z.I. Abramova // Kazan Medical Journal.- 2020.-V. 101, No. 3.- P.1-8	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=234180&p_lang=2	ru_RU
dc.description.abstract	Kazan Medical Journal	ru_RU
dc.description.abstract	Aim. To analyze the expression of key apoptosis (Bcl-2, caspase-3) and autophagy (Beclin 1, Vps34, p62 and LC3) proteins regulators in peripheral blood T-lymphocytes of patients with systemic lupus erythematosus.Methods. The object of the study was peripheral blood T-lymphocytes of healthy donors and patients with systemic lupus erythematosus. To obtain T cells, we used the immunomagnetic separation method. Protein expression was analyzed using the Western blot method. Statistically analyzing the results was performed using the R software environment. The data was represented using boxplots. Groups were compared using the Mann?Whitney test.Results. According to the results of the study of the apoptotic proteins, we found an increased content of caspase-3 and the absence of significant changes in the content of the anti-apoptotic protein Bcl-2 in patients with lupus, which indicates active apoptotic activity. A comparative analysis of Beclin 1 and Vps34 showed their increased content in the cells of patients, which indicates the activation of autophagy. The analysis of two isoforms of LC3 protein revealed their low content in the group of patients. Since the scatter of indicators was very different from the average value, we analyzed these indicators depending on the severity of the disease. In the acute course group, high content of protein LC3-I was detected, the content of form II was lower. In the group with the subacute course, the number of both isoforms is lower than in the other groups. In the group with a chronic course, significant increases of protein LC3-II and a decrease in the ratio of LC3-I/LC3-II were found.Conclusion. The study showed that depending on the severity of systemic lupus erythematosus, the content of protein LC3 isoforms changes, which can be used for differential diagnosis of disease forms.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Bcl-2	ru_RU
dc.subject	caspase-3	ru_RU
dc.subject	Beclin 1	ru_RU
dc.subject	Vps34	ru_RU
dc.subject	LC3	ru_RU
dc.subject	apoptosis	ru_RU
dc.subject	autophagy	ru_RU
dc.subject	systemic lupus erythematosus	ru_RU
dc.title	Induction of apoptosis and autophagy in T-lymphocytes of patients with Systemic Lupus Erythematosus	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU