KFU. Card publication. Pylotyping and genotyping of Escherichia coli isolates obtained from patients with colorectal cancer

KAZAN
FEDERAL UNIVERSITY

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PYLOTYPING AND GENOTYPING OF ESCHERICHIA COLI ISOLATES OBTAINED FROM PATIENTS WITH COLORECTAL CANCER

Form of presentation

Articles in international journals and collections

Year of publication

2020

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Authors, employees of KFU

Ilinskaya Olga Nikolaevna, author

Bibliographic description in the original language

Irina V. Rzhanova, Ramil R. Vafin, Tkhi Nga Nguyen, Alexey I. Kolpakov, Olga N. Ilinskaya. Pylotyping and genotyping of Escherichia coli isolates obtained from patients with colorectal cancer. International Journal of Pharmaceutical Research, Published on 01 Jul 2020 10.31838/ijpr/2020.12.03.076

Annotation

Objective of the research was to phylotype the E. coli isolates from the malignant and adjacent normal epithelium of the rectal mucosa of patients diagnosed with colorectal cancer, identifying and discriminating phylogroups by a set of genes, and also genotyping them by the fimH-gene locus by identifying fimH types. Samples of bacterial cultures isolated from paired biopsy specimens of patients diagnosed with colorectal cancer were subjected to molecular genetic research for species identification, as well as E. coli phylo- and genotyping. By sequencing the 16S rRNA locus, bacterial cultures are identified both as isolates of monocultures of E. coli and as mixed cultures of E. coli and K. pneumonia. The procedure for the phylotyping of E. coli isolates by identifying and discriminating phylogroups by a set of genes established the affiliation of samples of bacterial cultures with phylogroups A and B2. The genotyping procedure for E. coli isolates at the fimH gene locus by identifying the fimH types characterized the studied samples by signs of the third (f-3), fourth (f-4), and seventh (f-7) types, respectively. The strategy for identifying fimH-types during genotyping of E. coli at the fimH-gene locus has been improved, based on the analysis of SNP sequenced DNA sequences, and includes 52 fimH-types, three of which are justified in this paper.

Keywords

colorectal cancer, Escherichia coli, phylotyping, genotyping, fimH, PCR, sequencing, SNP

The name of the journal

International Journal of Pharmaceutical Research

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=235562&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Ilinskaya Olga Nikolaevna	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Irina V. Rzhanova, Ramil R. Vafin, Tkhi Nga Nguyen, Alexey I. Kolpakov, Olga N. Ilinskaya. Pylotyping and genotyping of Escherichia coli isolates obtained from patients with colorectal cancer. International Journal of Pharmaceutical Research, Published on 01 Jul 2020 10.31838/ijpr/2020.12.03.076	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=235562&p_lang=2	ru_RU
dc.description.abstract	International Journal of Pharmaceutical Research	ru_RU
dc.description.abstract	Objective of the research was to phylotype the E. coli isolates from the malignant and adjacent normal epithelium of the rectal mucosa of patients diagnosed with colorectal cancer, identifying and discriminating phylogroups by a set of genes, and also genotyping them by the fimH-gene locus by identifying fimH types. Samples of bacterial cultures isolated from paired biopsy specimens of patients diagnosed with colorectal cancer were subjected to molecular genetic research for species identification, as well as E. coli phylo- and genotyping. By sequencing the 16S rRNA locus, bacterial cultures are identified both as isolates of monocultures of E. coli and as mixed cultures of E. coli and K. pneumonia. The procedure for the phylotyping of E. coli isolates by identifying and discriminating phylogroups by a set of genes established the affiliation of samples of bacterial cultures with phylogroups A and B2. The genotyping procedure for E. coli isolates at the fimH gene locus by identifying the fimH types characterized the studied samples by signs of the third (f-3), fourth (f-4), and seventh (f-7) types, respectively. The strategy for identifying fimH-types during genotyping of E. coli at the fimH-gene locus has been improved, based on the analysis of SNP sequenced DNA sequences, and includes 52 fimH-types, three of which are justified in this paper.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	colorectal cancer	ru_RU
dc.subject	Escherichia coli	ru_RU
dc.subject	phylotyping	ru_RU
dc.subject	genotyping	ru_RU
dc.subject	fimH	ru_RU
dc.subject	PCR	ru_RU
dc.subject	sequencing	ru_RU
dc.subject	SNP	ru_RU
dc.title	Pylotyping and genotyping of Escherichia coli isolates obtained from patients with colorectal cancer	ru_RU
dc.type	Articles in international journals and collections	ru_RU