KFU. Card publication. Aureolic Acid Group of Agents as Potential Antituberculosis Drugs

KAZAN
FEDERAL UNIVERSITY

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AUREOLIC ACID GROUP OF AGENTS AS POTENTIAL ANTITUBERCULOSIS DRUGS

Form of presentation

Articles in international journals and collections

Year of publication

2020

Язык

английский

Authors, employees of KFU

Bespyatykh Andrey Vasilevich, author

Other authors

Ilina Elena , author

Klimina Ksenia , author

Malakhova Maja , author

Nikolenko Tatiana , author

Pozmogova Galina , author

Shitikov Egor , author

Tevyashova Anna , author

Varizhuk Anna , author

Bespyatykh Dmitriy Andreevich, author

Bespyatykh Yuliya Andreevna, author

Bibliographic description in the original language

Bespyatykh J., Bespiatykh D., Malakhova M., Klimina K., Bespyatykh A., Varizhuk A., Tevyashova A., Nikolenko T., Pozmogova G., Ilina E., Shitikov E. Aureolic Acid Group of Agents as Potential Antituberculosis Drugs //Antibiotics. – 2020. – T. 9. – №. 10. – R. 715-730.

Annotation

Mycobacterium tuberculosis is one of the most dangerous pathogens. Bacterial resistance to antituberculosis drugs grows each year, but searching for new drugs is a long process. Testing for available drugs to find active against mycobacteria may be a good alternative. In this work, antibiotics of the aureolic acid group were tested on a model organism Mycobacterium smegmatis. We presumed that antibiotics of this group may be potential G4 ligands. However, this was not confirmed in our analyses. We determined the antimicrobial activity of these drugs and revealed morphological changes in the cell structure upon treatment. Transcriptomic analysis documented increased expression of MSMEG_3743/soj and MSMEG_4228/ftsW, involved in cell division. Therefore, drugs may affect cell division, possibly disrupting the function of the Z-ring and the formation of a septum. Additionally, a decrease in the transcription level of several indispensable genes, such as nitrate reductase subunits (MSMEG_5137/narI and MSMEG_5139/narX) and MSMEG_3205/hisD was shown. We concluded that the mechanism of action of aureolic acid and its related compounds may be similar to that bedaquiline and disturb the NAD+/NADH balance in the cell. All of this allowed us to conclude that aureolic acid derivatives can be considered as potential antituberculosis drugs.

Keywords

Mycobacterium smegmatis; TB treatment; Olivomycin; Mycobacterium tuberculosis; transcriptomic

The name of the journal

Antibiotics

On-line resource for training course

http://dspace.kpfu.ru/xmlui/bitstream/handle/net/159521/F_antibiotics_09_00715_v2.pdf?sequence=1&isAllowed=y

URL

https://www.mdpi.com/2079-6382/9/10/715/pdf

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=240150&p_lang=2

Resource files

File name	Size (MB)	Format
F_antibiotics_09_00715_v2.pdf	1,15	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Bespyatykh Andrey Vasilevich	ru_RU
dc.contributor.author	Ilina Elena	ru_RU
dc.contributor.author	Klimina Ksenia	ru_RU
dc.contributor.author	Malakhova Maja	ru_RU
dc.contributor.author	Nikolenko Tatiana	ru_RU
dc.contributor.author	Pozmogova Galina	ru_RU
dc.contributor.author	Shitikov Egor	ru_RU
dc.contributor.author	Tevyashova Anna	ru_RU
dc.contributor.author	Varizhuk Anna	ru_RU
dc.contributor.author	Bespyatykh Dmitriy Andreevich	ru_RU
dc.contributor.author	Bespyatykh Yuliya Andreevna	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Bespyatykh J., Bespiatykh D., Malakhova M., Klimina K., Bespyatykh A., Varizhuk A., Tevyashova A., Nikolenko T., Pozmogova G., Ilina E., Shitikov E. Aureolic Acid Group of Agents as Potential Antituberculosis Drugs //Antibiotics. – 2020. – Т. 9. – №. 10. – Р. 715-730.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=240150&p_lang=2	ru_RU
dc.description.abstract	Antibiotics	ru_RU
dc.description.abstract	Mycobacterium tuberculosis is one of the most dangerous pathogens. Bacterial resistance to antituberculosis drugs grows each year, but searching for new drugs is a long process. Testing for available drugs to find active against mycobacteria may be a good alternative. In this work, antibiotics of the aureolic acid group were tested on a model organism Mycobacterium smegmatis. We presumed that antibiotics of this group may be potential G4 ligands. However, this was not confirmed in our analyses. We determined the antimicrobial activity of these drugs and revealed morphological changes in the cell structure upon treatment. Transcriptomic analysis documented increased expression of MSMEG_3743/soj and MSMEG_4228/ftsW, involved in cell division. Therefore, drugs may affect cell division, possibly disrupting the function of the Z-ring and the formation of a septum. Additionally, a decrease in the transcription level of several indispensable genes, such as nitrate reductase subunits (MSMEG_5137/narI and MSMEG_5139/narX) and MSMEG_3205/hisD was shown. We concluded that the mechanism of action of aureolic acid and its related compounds may be similar to that bedaquiline and disturb the NAD+/NADH balance in the cell. All of this allowed us to conclude that aureolic acid derivatives can be considered as potential antituberculosis drugs.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Aureolic Acid Group of Agents as Potential Antituberculosis Drugs	ru_RU
dc.type	Articles in international journals and collections	ru_RU