KFU. Card publication. Morpho-biochemical Characteristics of Autophagy of Lymphocytes from Peripheral Blood of Patients with Moderate and Severe Bronchial Asthma

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MORPHO-BIOCHEMICAL CHARACTERISTICS OF AUTOPHAGY OF LYMPHOCYTES FROM PERIPHERAL BLOOD OF PATIENTS WITH MODERATE AND SEVERE BRONCHIAL ASTHMA

Form of presentation

Articles in international journals and collections

Year of publication

2021

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Authors, employees of KFU

Abramov Sergey Nikolaevich, author

Abramova Zinaida Ivanovna, author

Evtyugin Vladimir Gennadevich, author

Reshetnikova Irina Dmitrievna, author

Skibo Yuliya Valerevna, author

Other authors

Vodounon Cyrille A -, author

Bibliographic description in the original language

Skibo. Y. V. Morpho-biochemical Characteristics of Autophagy of Lymphocytes from Peripheral Blood of Patients with Moderate and Severe Bronchial Asthma/ Y.V. Skibo, Cy.A. Vodounon, B. B. Legba, S. N. Abramov, V. G. Evtugyn, I. D. Reshetnikova, Zi. I. Abramova //American Journal of Biomedical Research.-2021.-V.9, N. 1.-P.10-18

Annotation

The increased interest in the process of autophagy in various physiological and pathological conditions is the focus of this study. Therefore, the aim was to assess the involvement of lymphocyte autophagy in the pathogenesis of bronchial asthma. Forty-five patients with mild persistent asthma, 45 patients with severe persistent asthma, and 45 normal healthy controls were involved in this study. Autophagy was evaluated based on the expression of microtubule-associated protein light chain 3 (LC3) by western blot, Fluorescence microscopy, and flow cytometry. Transmission Electron Microscopy was used to detect autophagosome. The results showed that autophagy was activated in T-cells of patients with mild and severe asthma compared to the normal healthy control. Stress conditions induced autophagy in T-cells of asthmatic patients with mild and severe form, but not in the control group. Dexamethasone treatment of T-cells stimulated apoptosis in mild asthma patients and in the control group but in the group with severe asthma, dexamethasone induced autophagy. Thus, autophagy could play an important role in the pathogenesis of asthma especially of severe asthma, and may contribute to the survival and the activation of T-lymphocyte in patients with severe asthma.

Keywords

autophagy, apoptosis, T lymphocytes, bronchial asthma

The name of the journal

American Journal of Biomedical Research

URL

https://pubs.sciepub.com/ajbr/9/1/3/index.html

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=252908&p_lang=2

Resource files

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autofagiya_ABA_American_Journal_of_Biomedical_Research.pdf	1,15	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Abramov Sergey Nikolaevich	ru_RU
dc.contributor.author	Abramova Zinaida Ivanovna	ru_RU
dc.contributor.author	Evtyugin Vladimir Gennadevich	ru_RU
dc.contributor.author	Reshetnikova Irina Dmitrievna	ru_RU
dc.contributor.author	Skibo Yuliya Valerevna	ru_RU
dc.contributor.author	Vodounon Cyrille A -	ru_RU
dc.date.accessioned	2021-01-01T00:00:00Z	ru_RU
dc.date.available	2021-01-01T00:00:00Z	ru_RU
dc.date.issued	2021	ru_RU
dc.identifier.citation	Skibo. Y. V. Morpho-biochemical Characteristics of Autophagy of Lymphocytes from Peripheral Blood of Patients with Moderate and Severe Bronchial Asthma/ Y.V. Skibo, Cy.A. Vodounon, B. B. Legba, S. N. Abramov, V. G. Evtugyn, I. D. Reshetnikova, Zi. I. Abramova //American Journal of Biomedical Research.-2021.-V.9, N. 1.-P.10-18	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=252908&p_lang=2	ru_RU
dc.description.abstract	American Journal of Biomedical Research	ru_RU
dc.description.abstract	The increased interest in the process of autophagy in various physiological and pathological conditions is the focus of this study. Therefore, the aim was to assess the involvement of lymphocyte autophagy in the pathogenesis of bronchial asthma. Forty-five patients with mild persistent asthma, 45 patients with severe persistent asthma, and 45 normal healthy controls were involved in this study. Autophagy was evaluated based on the expression of microtubule-associated protein light chain 3 (LC3) by western blot, Fluorescence microscopy, and flow cytometry. Transmission Electron Microscopy was used to detect autophagosome. The results showed that autophagy was activated in T-cells of patients with mild and severe asthma compared to the normal healthy control. Stress conditions induced autophagy in T-cells of asthmatic patients with mild and severe form, but not in the control group. Dexamethasone treatment of T-cells stimulated apoptosis in mild asthma patients and in the control group but in the group with severe asthma, dexamethasone induced autophagy. Thus, autophagy could play an important role in the pathogenesis of asthma especially of severe asthma, and may contribute to the survival and the activation of T-lymphocyte in patients with severe asthma.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	autophagy	ru_RU
dc.subject	apoptosis	ru_RU
dc.subject	T lymphocytes	ru_RU
dc.subject	bronchial asthma	ru_RU
dc.title	Morpho-biochemical Characteristics of Autophagy of Lymphocytes from Peripheral Blood of Patients with Moderate and Severe Bronchial Asthma	ru_RU
dc.type	Articles in international journals and collections	ru_RU