KFU. Card publication. Structural and Molecular Properties of Insect Type II Motor Axon Terminals.

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FEDERAL UNIVERSITY

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STRUCTURAL AND MOLECULAR PROPERTIES OF INSECT TYPE II MOTOR AXON TERMINALS.

Form of presentation

Articles in international journals and collections

Year of publication

2018

Язык

русский

Authors, employees of KFU

Biserova Natalya Mikhaylovna, author

Bibliographic description in the original language

Stocker B., Bochow C., Damrau C, Mathejczcyk T, Wolfenberg H., Colomb J., Weber C., Ramesh N., Duch C., Biserova NM., Sigrist S., Pflüger H.J. 2018. Structural and Molecular Properties of Insect Type II Motor Axon Terminals. // Frontiers in Systems Neuroscience. V.12. P1-16. doi: 10.3389/fnsys.2018.00005 [IF-3.928]

Annotation

A comparison between the axon terminals of octopaminergic efferent dorsal or ventral unpaired median neurons in either desert locusts (Schistocerca gregaria) or fruit flies (Drosophila melanogaster) across skeletal muscles reveals many similarities. In both species the octopaminergic axon forms beaded fibers where the boutons or varicosities form type II terminals in contrast to the neuromuscular junction (NMJ) or type I terminals. These type II terminals are immunopositive for both tyramine and octopamine and, in contrast to the type I terminals, which possess clear synaptic vesicles, only contain dense core vesicles. These dense core vesicles contain octopamine as shown by immunogold methods. With respect to the cytomatrix and active zone peptides the type II terminals exhibit active zone-like accumulations of the scaffold protein Bruchpilot (BRP) only sparsely in contrast to the many accumulations of BRP identifying active zones of NMJ type I terminals. In the fruit fly larva marked dynamic changes of octopaminergic fibers have been reported after short starvation which not only affects the formation of new branches (“synaptopods”) but also affects the type I terminals or NMJs via octopamine-signaling (Koon et al., 2011). Our starvation experiments of Drosophila-larvae revealed a time-dependency of the formation of additional branches. Whereas after 2 h of starvation we find a decrease in “synaptopods”, the increase is significant after 6 h of starvation. In addition, we provide evidence that the release of octopamine from dendritic and/or axonal type II terminals uses a similar synaptic machinery to glutamate release from type I terminals of excitatory motor neurons. Indeed, blocking this canonical synaptic release machinery via RNAi induced downregulation of BRP in neurons with type II terminals leads to flight performance deficits similar to those observed for octopamine mutants or flies lacking this class of neurons (Brembs et al., 2007).

Keywords

neuromuscular junction, Drosophila-larvae, Locust, ultrastructure, immuno-gold staining

The name of the journal

FRONTIERS IN SYSTEMS NEUROSCIENCE

URL

https://www.frontiersin.org/articles/10.3389/fnsys.2018.00005/full

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=264759&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Biserova Natalya Mikhaylovna	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	Stocker B., Bochow C., Damrau C, Mathejczcyk T, Wolfenberg H., Colomb J., Weber C., Ramesh N., Duch C., Biserova NM., Sigrist S., Pflüger H.J. 2018. Structural and Molecular Properties of Insect Type II Motor Axon Terminals. // Frontiers in Systems Neuroscience. V.12. P1-16. doi: 10.3389/fnsys.2018.00005 [IF-3.928]	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=264759&p_lang=2	ru_RU
dc.description.abstract	FRONTIERS IN SYSTEMS NEUROSCIENCE	ru_RU
dc.description.abstract	A comparison between the axon terminals of octopaminergic efferent dorsal or ventral unpaired median neurons in either desert locusts (Schistocerca gregaria) or fruit flies (Drosophila melanogaster) across skeletal muscles reveals many similarities. In both species the octopaminergic axon forms beaded fibers where the boutons or varicosities form type II terminals in contrast to the neuromuscular junction (NMJ) or type I terminals. These type II terminals are immunopositive for both tyramine and octopamine and, in contrast to the type I terminals, which possess clear synaptic vesicles, only contain dense core vesicles. These dense core vesicles contain octopamine as shown by immunogold methods. With respect to the cytomatrix and active zone peptides the type II terminals exhibit active zone-like accumulations of the scaffold protein Bruchpilot (BRP) only sparsely in contrast to the many accumulations of BRP identifying active zones of NMJ type I terminals. In the fruit fly larva marked dynamic changes of octopaminergic fibers have been reported after short starvation which not only affects the formation of new branches (“synaptopods”) but also affects the type I terminals or NMJs via octopamine-signaling (Koon et al., 2011). Our starvation experiments of Drosophila-larvae revealed a time-dependency of the formation of additional branches. Whereas after 2 h of starvation we find a decrease in “synaptopods”, the increase is significant after 6 h of starvation. In addition, we provide evidence that the release of octopamine from dendritic and/or axonal type II terminals uses a similar synaptic machinery to glutamate release from type I terminals of excitatory motor neurons. Indeed, blocking this canonical synaptic release machinery via RNAi induced downregulation of BRP in neurons with type II terminals leads to flight performance deficits similar to those observed for octopamine mutants or flies lacking this class of neurons (Brembs et al., 2007).	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	neuromuscular junction	ru_RU
dc.subject	Drosophila-larvae	ru_RU
dc.subject	Locust	ru_RU
dc.subject	ultrastructure	ru_RU
dc.subject	immuno-gold staining	ru_RU
dc.title	Structural and Molecular Properties of Insect Type II Motor Axon Terminals.	ru_RU
dc.type	Articles in international journals and collections	ru_RU