KFU. Card publication. Reappraisal of anoxic spreading depolarization as a terminal event during oxygen–glucose deprivation in brain slices in vitro

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REAPPRAISAL OF ANOXIC SPREADING DEPOLARIZATION AS A TERMINAL EVENT DURING OXYGEN&NDASH;GLUCOSE DEPRIVATION IN BRAIN SLICES IN VITRO

Form of presentation

Articles in international journals and collections

Year of publication

2020

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Authors, employees of KFU

Gaynutdinov Azat Robertovich, author

Mukhtarov Marat Rakhimzyanovich, author

Khazipov Rustem Narimanovich, author

Yuzekaeva Elvira Razilevna, author

Bibliographic description in the original language

Juzekaeva, E., Gainutdinov, A., Mukhtarov, M., & Khazipov, R. (2020). Reappraisal of anoxic spreading depolarization as a terminal event during oxygen–glucose deprivation in brain slices in vitro. Scientific Reports, 10(1), 18970. https://doi.org/10.1038/s41598-020-75975-w

Annotation

Scientific Reports |

Keywords

brain ischemia, hypoxia, edema, swelling, cortex, anoxic depolarization, spreading cortical depression, barrel cortex, barrel, optical intrinsic signals, silicone probes, electrophysiology

The name of the journal

Scientific Reports |

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=267210&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Gaynutdinov Azat Robertovich	ru_RU
dc.contributor.author	Mukhtarov Marat Rakhimzyanovich	ru_RU
dc.contributor.author	Khazipov Rustem Narimanovich	ru_RU
dc.contributor.author	Yuzekaeva Elvira Razilevna	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Juzekaeva, E., Gainutdinov, A., Mukhtarov, M., & Khazipov, R. (2020). Reappraisal of anoxic spreading depolarization as a terminal event during oxygen–glucose deprivation in brain slices in vitro. Scientific Reports, 10(1), 18970. https://doi.org/10.1038/s41598-020-75975-w	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=267210&p_lang=2	ru_RU
dc.description.abstract	Scientific Reports \|	ru_RU
dc.description.abstract	Anoxic spreading depolarization (aSD) has been hypothesized as a terminal event during oxygen– glucose deprivation (OGD) in submerged cortical slices in vitro. However, mechanical artifacts caused by aSD-triggered edema may introduce error in the assessment of neuronal viability. Here, using continuous patch-clamp recordings from submerged rat cortical slices, we first confirmed that vast majority of L4 neurons permanently lost their membrane potential during OGD-induced aSD. In some recordings, spontaneous transition from whole-cell to out-side out configuration occurred during or after aSD, and only a small fraction of neurons survived aSD with reperfusion started shortly after aSD. Secondly, to minimize artifacts caused by OGD-induced edema, cells were short-term patched following OGD episodes of various duration. Nearly half of L4 cells maintained membrane potential and showed the ability to spike-fire if reperfusion started less than 10 min after aSD. The probability of finding live neurons progressively decreased at longer reperfusion delays at a rate of about 2% per minute. We also found that neurons in L2/3 show nearly threefold higher resistance to OGD than neurons in L4. Our results suggest that in the OGD ischemia model, aSD is not a terminal event, and that the “commitment point” of irreversible damage occurs at variable delays, in the range of tens of minutes, after OGD-induced aSD in submerged cortical slices.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	brain ischemia	ru_RU
dc.subject	hypoxia	ru_RU
dc.subject	edema	ru_RU
dc.subject	swelling	ru_RU
dc.subject	cortex	ru_RU
dc.subject	anoxic depolarization	ru_RU
dc.subject	spreading cortical depression	ru_RU
dc.subject	barrel cortex	ru_RU
dc.subject	barrel	ru_RU
dc.subject	optical intrinsic signals	ru_RU
dc.subject	silicone probes	ru_RU
dc.subject	electrophysiology	ru_RU
dc.title	Reappraisal of anoxic spreading depolarization as a terminal event during oxygen–glucose deprivation in brain slices in vitro	ru_RU
dc.type	Articles in international journals and collections	ru_RU