KFU. Card publication. Bacterial Ribonuclease (Binase) Promotes Decontamination of MDBK Cell Cultures From Bovine Diarrhea Virus

KAZAN
FEDERAL UNIVERSITY

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BACTERIAL RIBONUCLEASE (BINASE) PROMOTES DECONTAMINATION OF MDBK CELL CULTURES FROM BOVINE DIARRHEA VIRUS

Form of presentation

Articles in international journals and collections

Year of publication

2021

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Authors, employees of KFU

Shakh Makhmud Raikhan , author

Bibliographic description in the original language

Efimova M. A.. Bacterial Ribonuclease (Binase) Promotes Decontamination of MDBK Cell Cultures From Bovine Diarrhea Virus / M. A.Efimova, A. G. Mukhamedzhanova, R. Shah Mahmud, N. I. Khammadov, K. V. Usoltcev, I. A. Nesterova, T. Kh. Faizov, O. N. Ilinskaya // BioNanoScience. - 2021.- T. 11. - P. 401–407.

Annotation

Bionanoscience

Keywords

Bovine virus, cell culture, enzyme

The name of the journal

Bionanoscience

URL

https://link.springer.com/article/10.1007/s12668-021-00862-1

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=271591&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Shakh Makhmud Raikhan	ru_RU
dc.date.accessioned	2021-01-01T00:00:00Z	ru_RU
dc.date.available	2021-01-01T00:00:00Z	ru_RU
dc.date.issued	2021	ru_RU
dc.identifier.citation	Efimova M. A.. Bacterial Ribonuclease (Binase) Promotes Decontamination of MDBK Cell Cultures From Bovine Diarrhea Virus / M. A.Efimova, A. G. Mukhamedzhanova, R. Shah Mahmud, N. I. Khammadov, K. V. Usoltcev, I. A. Nesterova, T. Kh. Faizov, O. N. Ilinskaya // BioNanoScience. - 2021.- T. 11. - P. 401–407.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=271591&p_lang=2	ru_RU
dc.description.abstract	Bionanoscience	ru_RU
dc.description.abstract	Mammalian cell cultivation is a technique that has played a crucial role in the development of biology, medicine, veterinary medicine, and pharmacology. Cell and tissue cultures are used as model systems in both the study of the mechanisms of pathogenesis of various diseases and the assessment of the effectiveness and toxicity of new drugs. They are frequently employed in the production of vaccines and biopharmaceuticals and play a part in assisted reproductive technologies. One of the factors that disrupt the stability of cell lines, thus affecting the results obtained and limiting the possibility of their application, is the contamination of cell cultures by various microorganisms, including bacteria, fungi, viruses, and mycoplasmas. According to a recent review, the bovine viral diarrhea virus (BVDV) is one of the most frequent and problematic contaminations occurring in animal and human cell cultures. Bacterial ribonuclease (binase) has shown considerable antiviral potential, and its activity against several viruses in vitro and in vivo has been reliably proven. In the present study, we present experimental results that demonstrate the role of bacterial ribonuclease (binase) in the contamination of cultures of bovine cell lines by cytopathogenic BVDV. According to these results, the treatment of infected Madin–Darby bovine kidney (MDBK) cells with binase led to a dose-dependent reduction in the level of viral RNA and infectious titer, thereby confirming the antiviral properties of the enzyme in vitro. Binase in concentrations of 100 to 300 μg/ml, which are nontoxic for cultures of MDBK cells, allowed to reduce the viral titer by 0.77 to 1.57 lg, correspondingly. Our results confirm the feasibility of using binase for the decontamination of cell cultures provided that the optimal modes of binase treatment are applied.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Bovine virus	ru_RU
dc.subject	cell culture	ru_RU
dc.subject	enzyme	ru_RU
dc.title	Bacterial Ribonuclease (Binase) Promotes Decontamination of MDBK Cell Cultures From Bovine Diarrhea Virus	ru_RU
dc.type	Articles in international journals and collections	ru_RU