KFU. Card publication. Optimization of β-1,4—glucosidase and acid phosphatase enzyme methods for agricultural soil studies

KAZAN
FEDERAL UNIVERSITY

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OPTIMIZATION OF β-1,4&MDASH;GLUCOSIDASE AND ACID PHOSPHATASE ENZYME METHODS FOR AGRICULTURAL SOIL STUDIES

Form of presentation

Conference proceedings in international journals and collections

Year of publication

2018

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Authors, employees of KFU

Galieva Gulnaz Shaykhinurovna, author

Galickaya Polina Yurevna, author

Selivanovskaya Svetlana Yurevna, author

Bibliographic description in the original language

Galieva G., Selivanovskaya S., Galitskaya P. Optimization of β-1,4—glucosidase and acid phosphatase enzyme methods for agricultural soil studies / Galieva G., Selivanovskaya S., Galitskaya P. // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM. International Multidisciplinary Scientific Geoconference, - 2018 . - Vol. 18, � 6.4. - P. 235–242.

Annotation

International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM

Keywords

β-1,4-glucosidase, Acid phosphatase, Buffer p�, Incubation time, Saturation curve, Soil microbial enzyme activities, Soil suspension, Synthetic fluorescent dye-conjugated substrates

The name of the journal

International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=274426&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Galieva Gulnaz Shaykhinurovna	ru_RU
dc.contributor.author	Galickaya Polina Yurevna	ru_RU
dc.contributor.author	Selivanovskaya Svetlana Yurevna	ru_RU
dc.date.accessioned	2018-01-01T00:00:00Z	ru_RU
dc.date.available	2018-01-01T00:00:00Z	ru_RU
dc.date.issued	2018	ru_RU
dc.identifier.citation	Galieva G., Selivanovskaya S., Galitskaya P. Optimization of β-1,4—glucosidase and acid phosphatase enzyme methods for agricultural soil studies / Galieva G., Selivanovskaya S., Galitskaya P. // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM. International Multidisciplinary Scientific Geoconference, - 2018 . - Vol. 18, � 6.4. - P. 235–242.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=274426&p_lang=2	ru_RU
dc.description.abstract	International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM	ru_RU
dc.description.abstract	In our study, we optimized two methods of enzyme activity estimations characterizing C and P cycles (β-1,4-glucosidase and acid phosphatase) for agricultural soil with 2.629 % of total C and 0.062 % of total N sampled in Tatarstan (Russia). Concentration of the substrates (4-MUF-β-d-glucopyranoside, 4-MUF-phosphate) in the range from 20 to 900 ?mol g-1 soil was used, four levels of buffer pH (3, 6, 8, 11) were compared, and three different dilutions of soil suspension (1%, 2%, 10% w/v) were investigated. Besides, the optimal time of measurement was chosen. For β-1,4-glucosidase, it was revealed that 4-MUB-β-d-glucopyranoside concentration should be 400 ?mol g-1 of soil, buffer pH should be 8.0, and soil suspension should be prepared using 1 part of soil and 100 parts of water. The measurements should be done after 60 min of incubation. For acid phosphatase, it was revealed that 4-MUB-phosphate concentration should be 400 ?mol g-1 of soil, buffer pH should be 11.0, and soil suspension should be prepared using 1 part of soil and 50 parts of water. The measurements should be done after 30 min of incubation. ? 2018, International Multidisciplinary Scientific Geoconference. All rights reserved.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	β-1	ru_RU
dc.subject	4-glucosidase	ru_RU
dc.subject	Acid phosphatase	ru_RU
dc.subject	Buffer p�	ru_RU
dc.subject	Incubation time	ru_RU
dc.subject	Saturation curve	ru_RU
dc.subject	Soil microbial enzyme activities	ru_RU
dc.subject	Soil suspension	ru_RU
dc.subject	Synthetic fluorescent dye-conjugated substrates	ru_RU
dc.title	Optimization of β-1,4—glucosidase and acid phosphatase enzyme methods for agricultural soil studies	ru_RU
dc.type	Conference proceedings in international journals and collections	ru_RU