KFU. Card publication. Human mesenchymal stem cells overexpressing interleukin 2 can suppress proliferation of neuroblastoma cells in co-culture and activate mononuclear cells in vitro

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HUMAN MESENCHYMAL STEM CELLS OVEREXPRESSING INTERLEUKIN 2 CAN SUPPRESS PROLIFERATION OF NEUROBLASTOMA CELLS IN CO-CULTURE AND ACTIVATE MONONUCLEAR CELLS IN VITRO

Form of presentation

Articles in international journals and collections

Year of publication

2020

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Authors, employees of KFU

Arkhipova Svetlana Sergeevna, author

Akhmetzyanova Elvira Ruslanovna, author

Garanina Ekaterina Evgenevna, author

Gomzikova Marina Olegovna, author

Rizvanov Albert Anatolevich, author

Soloveva Valeriya Vladimirovna, author

Tazetdinova Leysan Gazinurovna, author

Khaybullina Svetlana Francevna, author

Chulpanova Darya Sergeevna, author

Other authors

Dzheyms Viktoriya , author

Bibliographic description in the original language

Chulpanova, D. S., Solovyeva, V. V., James, V., Arkhipova, S. S., Gomzikova, M. O., Garanina, E. E., Akhmetzyanova, E.R., Tazetdinova, L.G., Khaiboullina, S.F. & Rizvanov, A. A. (2020). Human mesenchymal stem cells overexpressing interleukin 2 can suppress proliferation of neuroblastoma cells in co-culture and activate mononuclear cells in vitro. Bioengineering, 7(2), 59.

Annotation

Bioengineering

Keywords

immunotherapy; mesenchymal stem cells; interleukin 2; cancer therapy; neuroblastoma

The name of the journal

Bioengineering

URL

https://www.mdpi.com/2306-5354/7/2/59

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=284382&p_lang=2

Resource files

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bioengineering_07_00059.pdf	4,03	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Arkhipova Svetlana Sergeevna	ru_RU
dc.contributor.author	Akhmetzyanova Elvira Ruslanovna	ru_RU
dc.contributor.author	Garanina Ekaterina Evgenevna	ru_RU
dc.contributor.author	Gomzikova Marina Olegovna	ru_RU
dc.contributor.author	Rizvanov Albert Anatolevich	ru_RU
dc.contributor.author	Soloveva Valeriya Vladimirovna	ru_RU
dc.contributor.author	Tazetdinova Leysan Gazinurovna	ru_RU
dc.contributor.author	Khaybullina Svetlana Francevna	ru_RU
dc.contributor.author	Chulpanova Darya Sergeevna	ru_RU
dc.contributor.author	Dzheyms Viktoriya	ru_RU
dc.date.accessioned	2020-01-01T00:00:00Z	ru_RU
dc.date.available	2020-01-01T00:00:00Z	ru_RU
dc.date.issued	2020	ru_RU
dc.identifier.citation	Chulpanova, D. S., Solovyeva, V. V., James, V., Arkhipova, S. S., Gomzikova, M. O., Garanina, E. E., Akhmetzyanova, E.R., Tazetdinova, L.G., Khaiboullina, S.F. & Rizvanov, A. A. (2020). Human mesenchymal stem cells overexpressing interleukin 2 can suppress proliferation of neuroblastoma cells in co-culture and activate mononuclear cells in vitro. Bioengineering, 7(2), 59.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=284382&p_lang=2	ru_RU
dc.description.abstract	Bioengineering	ru_RU
dc.description.abstract	High-dose recombinant interleukin 2 (IL2) therapy has been shown to be successful in renal cell carcinoma and metastatic melanoma. However, systemic administration of high doses of IL2 can be toxic, causing capillary leakage syndrome and stimulating pro-tumor immune response. One of the strategies to reduce the systemic toxicity of IL2 is the use of mesenchymal stem cells (MSCs) as a vehicle for the targeted delivery of IL2. Human adipose tissue-derived MSCs were transduced with lentivirus encoding IL2 (hADSCs-IL2) or blue fluorescent protein (BFP) (hADSCs-BFP). The proliferation, immunophenotype, cytokine profile and ultrastructure of hADSCs-IL2 and hADSCs-BFP were determined. The effect of hADSCs on activation of peripheral blood mononuclear cells (PBMCs) and proliferation and viability of SH-SY5Y neuroblastoma cells after co-culture with native hADSCs, hADSCs-BFP or hADSCs-IL2 on plastic and Matrigel was evaluated. Ultrastructure and cytokine production by hADSCs-IL2 showed modest changes in comparison with hADSCs and hADSCs-BFP. Conditioned medium from hADSC-IL2 affected tumor cell proliferation, increasing the proliferation of SH-SY5Y cells and also increasing the number of late-activated T-cells, natural killer (NK) cells, NKT-cells and activated T-killers. Conversely, hADSC-IL2 co-culture led to a decrease in SH-SY5Y proliferation on plastic and Matrigel. These data show that hADSCs-IL2 can reduce SH-SY5Y proliferation and activate PBMCs in vitro. However, IL2-mediated therapeutic effects of hADSCs could be offset by the increased expression of pro-oncogenes, as well as the natural ability of hADSCs to promote the progression of some tumors.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Human mesenchymal stem cells overexpressing interleukin 2 can suppress proliferation of neuroblastoma cells in co-culture and activate mononuclear cells in vitro	ru_RU
dc.type	Articles in international journals and collections	ru_RU