Form of presentation | Articles in international journals and collections |
Year of publication | 2024 |
Язык | английский |
|
Bulatov Emil Rafaelevich, author
Kiyamov Ayrat Gazinurovich, author
Miftakhova Regina Rifkatovna, author
Rizvanov Albert Anatolevich, author
Filina Yuliya Viktorovna, author
|
Bibliographic description in the original language |
Rakhmatullina A.R, Zolotykh M.A, Filina Y.V, Development of a novel prostate Cancer-Stroma Sphere (CSS) model for In Vitro tumor microenvironment studies//Translational Oncology. - 2024. - Vol.44, Is.. - Art. №101930. |
Annotation |
Tumor employs non-cancerous cells to gain beneficial features that promote growth and survival of cancer cells. Despite intensive research in the area of tumor microenvironment, there is still a lack of reliable and reproducible in vitro model for tumor and tumor-microenvironment cell interaction studies. Herein we report the successful development of a heterogeneous cancer-stroma sphere (CSS) model composed of prostate adenocarcinoma PC3 cells and immortalized mesenchymal stem cells (MSC). The CSS model demonstrated a structured spatial layout of the cells, with stromal cells concentrated at the center of the spheres and tumor cells located on the periphery. Significant increase in the levels of VEGFA, IL-10, and IL1a has been detected in the conditioned media of CSS as compared to PC3 spheres. Single cell RNA sequencing data revealed that VEGFA was secreted by MSC cells within heterogeneous spheroids. Enhanced expression of extracellular membrane (ECM) proteins was also shown for CSS-derived MSCs. Furthermore, we demonstrated that the multicellular architecture altered cancer cell response to chemotherapeutic agents: the inhibition of sphere formation by topotecan was 74.92 ? 4.56 % for PC3 spheres and 45.95 ? 7.84 % for CSS spheres (p < 0.01), docetaxel showed 37,51? 20,88 % and 15,67? 14,08 % inhibition, respectively (p < 0.05). Thus, CSS present an effective in vitro model for examining the extracellular matrix composition and cell-to-cell interactions within the tumor, as well as for evaluating the antitumor activity of drugs. |
Keywords |
mesenchymal stem cells, tumor microenviroment |
The name of the journal |
Translational Oncology
|
URL |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85188605826&doi=10.1016%2fj.tranon.2024.101930&partnerID=40&md5=72e360ac3eb5e786914989676594291d |
Please use this ID to quote from or refer to the card |
https://repository.kpfu.ru/eng/?p_id=298201&p_lang=2 |
Full metadata record ![](https://shelly.kpfu.ru/pdf/picture/arrow_black_right.gif) |
Field DC |
Value |
Language |
dc.contributor.author |
Bulatov Emil Rafaelevich |
ru_RU |
dc.contributor.author |
Kiyamov Ayrat Gazinurovich |
ru_RU |
dc.contributor.author |
Miftakhova Regina Rifkatovna |
ru_RU |
dc.contributor.author |
Rizvanov Albert Anatolevich |
ru_RU |
dc.contributor.author |
Filina Yuliya Viktorovna |
ru_RU |
dc.date.accessioned |
2024-01-01T00:00:00Z |
ru_RU |
dc.date.available |
2024-01-01T00:00:00Z |
ru_RU |
dc.date.issued |
2024 |
ru_RU |
dc.identifier.citation |
Rakhmatullina A.R, Zolotykh M.A, Filina Y.V, Development of a novel prostate Cancer-Stroma Sphere (CSS) model for In Vitro tumor microenvironment studies//Translational Oncology. - 2024. - Vol.44, Is.. - Art. №101930. |
ru_RU |
dc.identifier.uri |
https://repository.kpfu.ru/eng/?p_id=298201&p_lang=2 |
ru_RU |
dc.description.abstract |
Translational Oncology |
ru_RU |
dc.description.abstract |
Tumor employs non-cancerous cells to gain beneficial features that promote growth and survival of cancer cells. Despite intensive research in the area of tumor microenvironment, there is still a lack of reliable and reproducible in vitro model for tumor and tumor-microenvironment cell interaction studies. Herein we report the successful development of a heterogeneous cancer-stroma sphere (CSS) model composed of prostate adenocarcinoma PC3 cells and immortalized mesenchymal stem cells (MSC). The CSS model demonstrated a structured spatial layout of the cells, with stromal cells concentrated at the center of the spheres and tumor cells located on the periphery. Significant increase in the levels of VEGFA, IL-10, and IL1a has been detected in the conditioned media of CSS as compared to PC3 spheres. Single cell RNA sequencing data revealed that VEGFA was secreted by MSC cells within heterogeneous spheroids. Enhanced expression of extracellular membrane (ECM) proteins was also shown for CSS-derived MSCs. Furthermore, we demonstrated that the multicellular architecture altered cancer cell response to chemotherapeutic agents: the inhibition of sphere formation by topotecan was 74.92 ? 4.56 % for PC3 spheres and 45.95 ? 7.84 % for CSS spheres (p < 0.01), docetaxel showed 37,51? 20,88 % and 15,67? 14,08 % inhibition, respectively (p < 0.05). Thus, CSS present an effective in vitro model for examining the extracellular matrix composition and cell-to-cell interactions within the tumor, as well as for evaluating the antitumor activity of drugs. |
ru_RU |
dc.language.iso |
ru |
ru_RU |
dc.subject |
mesenchymal stem cells |
ru_RU |
dc.subject |
tumor microenviroment |
ru_RU |
dc.title |
Development of a novel prostate Cancer-Stroma Sphere (CSS) model for In Vitro tumor microenvironment studies |
ru_RU |
dc.type |
Articles in international journals and collections |
ru_RU |
|