KFU. Card publication. ВЛИЯНИЕ ОКИСЛИТЕЛЬНОГО СТРЕССА НА АКТИВНОСТЬ КИНАЗЫ mTOR В CD4+ Т-ЛИМФОЦИТАХ ПРИ АУТОИММУННОМ ТИРЕОИДИТЕ

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ВЛИЯНИЕ ОКИСЛИТЕЛЬНОГО СТРЕССА НА АКТИВНОСТЬ КИНАЗЫ MTOR В CD4+ Т-ЛИМФОЦИТАХ ПРИ АУТОИММУННОМ ТИРЕОИДИТЕ

Form of presentation

Articles in Russian journals and collections

Year of publication

2025

Язык

русский

Authors, employees of KFU

Abramova Zinaida Ivanovna, author

Other authors

Afanaseva Zinaida Aleksandrovna, author

Authors, students KFU

Burceva Anastasiya Vladimirovna, author

Tikhonova Anastasiya Nikolaevna, author

Bibliographic description in the original language

Burceva A.V. Vliyanie okislitelnogo stressa na aktivnost faktorov apoptoza i autofagii svyazannykh s mitokhondriyami CD4+T limfocitov pri autoimmunnom tireoidite / A.V Burceva, A.N.Tikhonova, Z. A. Afanaseva, Z.I. Abramova // Medicinskaya immunologiya.-2025.-T.27,№ 4.-S.847-862

Annotation

Current studies of autoimmunity processes are addressing specific processes involving mitochondria, i.e., altered intracellular signaling including the generation of ROS and release of proteins from mitochondria to the cytoplasm, thus activating apoptosis. These functions of mitochondria are usually associated with disturbed bioenergetic functions and excessive production of H2O2. Currently, a new area is actively developing, i.e., potential participation of mitochondria in the immune response. Therefore, the aim of the present study was to evaluate the dynamics of apoptosis factors and autophagy associated with mitochondria of CD4+T cells in autoimmune thyroiditis (AIT). The study was performed with CD4+T cells of AIT patients and healthy donors obtained by magnetic separation. Apoptosis and autophagy were assessed by flow cytometry. Western blotting was used to analyze autophagy marker proteins (p62, LC3I/II, mTOR kinase), and apo ptosis regulator Bcl- 2 in CD4+T lymphocytes of the patients and donors. The state of mitochondria in CD4+T lymphocytes was assessed by confocal microscopy. Using an experimental model with increased H2O2 , we showed that ROS activate LC3 protein in cells of patients with AIT along with accumulation of autophagic adapter protein p62, as registered on the outer mitochondrial membrane. An increased level of mitophagoses was found in CD4+T cells from the AIT patients. On the basis of these data, one may assume that H2O2 causes activation of mitophagy in CD4+T cells of patients with AIT, and the development of oxidative stress with excessive production of ROS leads to irreversible damage to mitochondria, which causes a decreased apoptotic activity followed by development of secondary necrosis of CD4+T lymphocytes in AIT, making them cytotoxic. Accumulation of such cells in the thyroid tissue may lead to impaired apoptosis in thyrocytes and, as a consequence, to secondary necrosis, thus resulting in development of autoimmune response. The obtained data require additional studies, since the detection of CD4+T lymphocytes prone for apoptosis or necrosis in patients with AIT may be used as a diagnostic criterion for prediction of inflammatory conditions.

Keywords

окислительный стресс, mTOR, CD4+Т-лимфоцит, митофагия, аутоиммунном тиреоидит

The name of the journal

Медицинская иммунология

URL

https://doi.org/10.15789/1563-0625-IOO-3141

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=312467&p_lang=2

Resource files

File name	Size (MB)	Format
F_VLIYaNIE_OKISLITELNOGO_STRESSA_NA_AKTIVNOST..._AIT.pdf	0,45	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Abramova Zinaida Ivanovna	ru_RU
dc.contributor.author	Afanaseva Zinaida Aleksandrovna	ru_RU
dc.contributor.author	Burceva Anastasiya Vladimirovna	ru_RU
dc.contributor.author	Tikhonova Anastasiya Nikolaevna	ru_RU
dc.date.accessioned	2025-01-01T00:00:00Z	ru_RU
dc.date.available	2025-01-01T00:00:00Z	ru_RU
dc.date.issued	2025	ru_RU
dc.identifier.citation	Бурцева А.В. Влияние окислительного стресса на активность факторов апоптоза и аутофагии связанных с митохондриями CD4+Т лимфоцитов при аутоиммунном тиреоидите / А.В Бурцева, А.Н.Тихонова, З. А. Афанасьева, З.И. Абрамова // Медицинская иммунология.-2025.-Т.27,№ 4.-С.847-862	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=312467&p_lang=2	ru_RU
dc.description.abstract	Медицинская иммунология	ru_RU
dc.description.abstract	Current studies of autoimmunity processes are addressing specific processes involving mitochondria, i.e., altered intracellular signaling including the generation of ROS and release of proteins from mitochondria to the cytoplasm, thus activating apoptosis. These functions of mitochondria are usually associated with disturbed bioenergetic functions and excessive production of H2O2. Currently, a new area is actively developing, i.e., potential participation of mitochondria in the immune response. Therefore, the aim of the present study was to evaluate the dynamics of apoptosis factors and autophagy associated with mitochondria of CD4+T cells in autoimmune thyroiditis (AIT). The study was performed with CD4+T cells of AIT patients and healthy donors obtained by magnetic separation. Apoptosis and autophagy were assessed by flow cytometry. Western blotting was used to analyze autophagy marker proteins (p62, LC3I/II, mTOR kinase), and apo ptosis regulator Bcl- 2 in CD4+T lymphocytes of the patients and donors. The state of mitochondria in CD4+T lymphocytes was assessed by confocal microscopy. Using an experimental model with increased H2O2 , we showed that ROS activate LC3 protein in cells of patients with AIT along with accumulation of autophagic adapter protein p62, as registered on the outer mitochondrial membrane. An increased level of mitophagoses was found in CD4+T cells from the AIT patients. On the basis of these data, one may assume that H2O2 causes activation of mitophagy in CD4+T cells of patients with AIT, and the development of oxidative stress with excessive production of ROS leads to irreversible damage to mitochondria, which causes a decreased apoptotic activity followed by development of secondary necrosis of CD4+T lymphocytes in AIT, making them cytotoxic. Accumulation of such cells in the thyroid tissue may lead to impaired apoptosis in thyrocytes and, as a consequence, to secondary necrosis, thus resulting in development of autoimmune response. The obtained data require additional studies, since the detection of CD4+T lymphocytes prone for apoptosis or necrosis in patients with AIT may be used as a diagnostic criterion for prediction of inflammatory conditions.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	окислительный стресс	ru_RU
dc.subject	mTOR	ru_RU
dc.subject	CD4+Т-лимфоцит	ru_RU
dc.subject	митофагия	ru_RU
dc.subject	аутоиммунном тиреоидит	ru_RU
dc.title	ВЛИЯНИЕ ОКИСЛИТЕЛЬНОГО СТРЕССА НА АКТИВНОСТЬ КИНАЗЫ mTOR В CD4+ Т-ЛИМФОЦИТАХ ПРИ АУТОИММУННОМ ТИРЕОИДИТЕ	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU