KFU. Card publication. Stimulation of Alpha-2 Adrenoreceptors against the Background of Hyperpolarization-Activated Current Blockade at Different Formation Stages of Cardiac Adrenergic Innervation

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STIMULATION OF ALPHA-2 ADRENORECEPTORS AGAINST THE BACKGROUND OF HYPERPOLARIZATION-ACTIVATED CURRENT BLOCKADE AT DIFFERENT FORMATION STAGES OF CARDIAC ADRENERGIC INNERVATION

Form of presentation

Articles in Russian journals and collections

Year of publication

2025

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Authors, employees of KFU

Zefirov Timur Lvovich, author

Ziyatdinova Nafisa Ilgizovna, author

Iskakov Nikita Georgievich, author

Kupcova Anna Mikhaylovna, author

Other authors

Zefirov Andrey Lvovich, author

Bibliographic description in the original language

Kuptsova A.M. Stimulation of Alpha-2 Adrenoreceptors against the Background of Hyperpolarization-Activated Current Blockade at Different Formation Stages of Cardiac Adrenergic Innervation /A.M. Kuptsova, N.I. Ziyatdinova, A.L. Zefirov, N.G. Iskakov, T.L. Zefirov // Journal of Evolutionary Biochemistry and Physiology. 2025. - Vol. 61. - N. 5. - pp. 1363–1371.

Annotation

Abstract—In different periods of postnatal development, the components of the autonomic nervous system, including specific receptors and neurotransmitters involved in the control of cardiovascular functions, are characterized by significant dynamics and variability. Although in humans and animals, α2-adrenoceptors (α2-ARs) and the hyperpolarization-activated current (If) are widespread and involved in the regulation of multiple functions, their modulatory role at different stages of the formation and development of cardiac adrenergic regulation has been insufficiently studied. The present study was carried out on isolated hearts of 1-week-old, 3-week-old, and adult white rats. Heart rate (HR) and coronary flow (CF) in the Langendorff rat heart were recorded upon α2-AR activation with clonidine hydrochloride (10–6 M) against the background of If blocker ZD7288 application at 10–9 M and 10–5 M. In 1-week-old rat pups, α2-ARs activation evoked bidirectional changes in CF depending on If blocker concentration, causing its significant increase at 10–9 M and bidirectional dynamics at 10–5 M. In 3-week-old rat pups, α2-AR stimulation reduced CF, while against the background of preliminary If blockade, the CF dynamics changed to positive. In adult rats, α2-AR activation against the background of If blockade caused a decrease in CF. The α2-AR agonist evoked bidirectional changes in HR in 1-week-old and adult rats, causing only a significant reduction in HR in 3-week-old rat pups. Stimulation of α2-ARs against the background of If blockade did not alter the HR dynamics in 3-week-old rats and abolished bidirectional HR changes in adult and 1-week-old animals, causing only a decrease in HR in adults and an increase in HR in newborns. The obtained results demonstrate that α2-adrenergic cardiac regulation is modulated by If, with the degree and direction of the effects depending on the maturity level of cardiac sympathetic innervation.

Keywords

alpha-2 adrenergic receptor, hyperpolarization-activated current, isolated heart, ontogenesis, rat

The name of the journal

JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY

URL

https://link.springer.com/article/10.1134/S0022093025050047?utm_source=rct_congratemailt&utm_medium=email&utm_campaign=nonoa_20251105&utm_content=10.1134/S0022093025050047

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=319155&p_lang=2

Resource files

File name	Size (MB)	Format
2025_RFZh_im._Sechenova.pdf	0,55	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Zefirov Timur Lvovich	ru_RU
dc.contributor.author	Ziyatdinova Nafisa Ilgizovna	ru_RU
dc.contributor.author	Iskakov Nikita Georgievich	ru_RU
dc.contributor.author	Kupcova Anna Mikhaylovna	ru_RU
dc.contributor.author	Zefirov Andrey Lvovich	ru_RU
dc.date.accessioned	2025-01-01T00:00:00Z	ru_RU
dc.date.available	2025-01-01T00:00:00Z	ru_RU
dc.date.issued	2025	ru_RU
dc.identifier.citation	Kuptsova A.M. Stimulation of Alpha-2 Adrenoreceptors against the Background of Hyperpolarization-Activated Current Blockade at Different Formation Stages of Cardiac Adrenergic Innervation /A.M. Kuptsova, N.I. Ziyatdinova, A.L. Zefirov, N.G. Iskakov, T.L. Zefirov // Journal of Evolutionary Biochemistry and Physiology. 2025. - Vol. 61. - N. 5. - pp. 1363–1371.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=319155&p_lang=2	ru_RU
dc.description.abstract	JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY	ru_RU
dc.description.abstract	Abstract—In different periods of postnatal development, the components of the autonomic nervous system, including specific receptors and neurotransmitters involved in the control of cardiovascular functions, are characterized by significant dynamics and variability. Although in humans and animals, α2-adrenoceptors (α2-ARs) and the hyperpolarization-activated current (If) are widespread and involved in the regulation of multiple functions, their modulatory role at different stages of the formation and development of cardiac adrenergic regulation has been insufficiently studied. The present study was carried out on isolated hearts of 1-week-old, 3-week-old, and adult white rats. Heart rate (HR) and coronary flow (CF) in the Langendorff rat heart were recorded upon α2-AR activation with clonidine hydrochloride (10–6 M) against the background of If blocker ZD7288 application at 10–9 M and 10–5 M. In 1-week-old rat pups, α2-ARs activation evoked bidirectional changes in CF depending on If blocker concentration, causing its significant increase at 10–9 M and bidirectional dynamics at 10–5 M. In 3-week-old rat pups, α2-AR stimulation reduced CF, while against the background of preliminary If blockade, the CF dynamics changed to positive. In adult rats, α2-AR activation against the background of If blockade caused a decrease in CF. The α2-AR agonist evoked bidirectional changes in HR in 1-week-old and adult rats, causing only a significant reduction in HR in 3-week-old rat pups. Stimulation of α2-ARs against the background of If blockade did not alter the HR dynamics in 3-week-old rats and abolished bidirectional HR changes in adult and 1-week-old animals, causing only a decrease in HR in adults and an increase in HR in newborns. The obtained results demonstrate that α2-adrenergic cardiac regulation is modulated by If, with the degree and direction of the effects depending on the maturity level of cardiac sympathetic innervation.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	alpha-2 adrenergic receptor	ru_RU
dc.subject	hyperpolarization-activated current	ru_RU
dc.subject	isolated heart	ru_RU
dc.subject	ontogenesis	ru_RU
dc.subject	rat	ru_RU
dc.title	Stimulation of Alpha-2 Adrenoreceptors against the Background of Hyperpolarization-Activated Current Blockade at Different Formation Stages of Cardiac Adrenergic Innervation	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU