KFU. Card publication. Rapid identification of Lactobacillus plantarum by RAPD-PCR

KAZAN
FEDERAL UNIVERSITY

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RAPID IDENTIFICATION OF LACTOBACILLUS PLANTARUM BY RAPD-PCR

Form of presentation

Articles in international journals and collections

Year of publication

1998

Язык

русский

Authors, employees of KFU

Validov Shamil Zavdatovich, author

Bibliographic description in the original language

Validov, S. Identification, Lactobacillus plantarum, Monitoring, RAPD-PCR, Silage, Species / Pan'kova, N.V., Kozlova, E.V., Kuz'min, N.P., Klimenko, V.V., Boronin, A.M Microbiology. – 1998. 68- №3. – S. 317 - 322. – Bibliogr. : s. 317.

Annotation

The wide distribution and practical use of lactobacilli necessitates development of methods for their rapid isolation and identification. In particular, such methods are necessary for monitoring natural and introduced strains of lactobacilli during ensilage to optimize this process. Twelve strains of lactobacilli were isolated from silage and wheat rhizosphere on Rogosa medium. The polymerase chain reaction with arbitrary primer (random amplified polymorphic DNA polymerase chain reaction, RAPD-PCR) was used for species identification of the isolates and collection strains. A 17-nucleotide-long primer designated pUC/M13 was used. The patterns of amplification products often isolates were found to be similar to those of the collection strains of Lactobacillus plantarum, indicating that they belong to this species. A study of physiological and biochemical characteristics (growth at different temperatures and the profile of fermented carbohydrates) confirmed conclusions based on the result

Keywords

Identification, Lactobacillus plantarum, Monitoring, RAPD-PCR, Silage, Species

The name of the journal

Микробиология

URL

http://www.scopus.com/source/sourceInfo.url?sourceId=20269&origin=recordpage

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=80361&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Validov Shamil Zavdatovich	ru_RU
dc.date.accessioned	1998-01-01T00:00:00Z	ru_RU
dc.date.available	1998-01-01T00:00:00Z	ru_RU
dc.date.issued	1998	ru_RU
dc.identifier.citation	Validov, S. Identification, Lactobacillus plantarum, Monitoring, RAPD-PCR, Silage, Species / Pan'kova, N.V., Kozlova, E.V., Kuz'min, N.P., Klimenko, V.V., Boronin, A.M Microbiology. – 1998. 68- №3. – С. 317 - 322. – Библиогр. : с. 317.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=80361&p_lang=2	ru_RU
dc.description.abstract	Микробиология	ru_RU
dc.description.abstract	The wide distribution and practical use of lactobacilli necessitates development of methods for their rapid isolation and identification. In particular, such methods are necessary for monitoring natural and introduced strains of lactobacilli during ensilage to optimize this process. Twelve strains of lactobacilli were isolated from silage and wheat rhizosphere on Rogosa medium. The polymerase chain reaction with arbitrary primer (random amplified polymorphic DNA polymerase chain reaction, RAPD-PCR) was used for species identification of the isolates and collection strains. A 17-nucleotide-long primer designated pUC/M13 was used. The patterns of amplification products often isolates were found to be similar to those of the collection strains of Lactobacillus plantarum, indicating that they belong to this species. A study of physiological and biochemical characteristics (growth at different temperatures and the profile of fermented carbohydrates) confirmed conclusions based on the result	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Identification	ru_RU
dc.subject	Lactobacillus plantarum	ru_RU
dc.subject	Monitoring	ru_RU
dc.subject	RAPD-PCR	ru_RU
dc.subject	Silage	ru_RU
dc.subject	Species	ru_RU
dc.title	Rapid identification of Lactobacillus plantarum by RAPD-PCR	ru_RU
dc.type	Articles in international journals and collections	ru_RU