KFU. Card publication. Cell-Penetrating Nanobiosensors for Pointillistic Intracellular Ca2+-Transient Detection

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FEDERAL UNIVERSITY

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CELL-PENETRATING NANOBIOSENSORS FOR POINTILLISTIC INTRACELLULAR CA2+-TRANSIENT DETECTION

Form of presentation

Articles in international journals and collections

Year of publication

2014

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Authors, employees of KFU

Yakovlev Aleksey Valerevich, author

Bibliographic description in the original language

Alsu I. Zamaleeva, Mayeul Collot, Eloi Bahembera, Céline Tisseyre, Philippe Rostaing, Aleksey V. Yakovlev, Martin Oheim, Michel de Waard, Jean-Maurice Mallet, and Anne Feltz Nano Letters 2014 14 (6), 2994-3001 DOI: 10.1021/nl500733g

Annotation

Small-molecule chemical calcium (Ca2+) indicators are invaluable tools for studying intracellular signaling pathways but have severe shortcomings for detecting local Ca2+ entry. Nanobiosensors incorporating functionalized quantum dots (QDs) have emerged as promising alternatives but their intracellular use remains a major challenge. We designed cell-penetrating FRET-based Ca2+ nanobiosensors for the detection of local Ca2+ concentration transients, using commercially available CANdot565QD as a donor and CaRuby, a custom red-emitting Ca2+ indicator, as an acceptor. With Ca2+-binding affinities covering the range of 3?20 μM, our CaRubies allow building sensors with a scalable affinity for detecting intracellular Ca2+ transients at various concentrations. To facilitate their cytoplasmic delivery, QDs were further functionalized with a small cell-penetrating peptide (CPP) derived from hadrucalcin (HadUF1?11: H11), a ryanodine receptor-directed scorpion toxin identified within the venom of

Keywords

Quantum dot biosensors; nanoparticle surface chemistry; FRET-based calcium probes; red-emitting calcium indicator; intracellular calcium fluorimetry; cell penetrating peptide

The name of the journal

Nano Letters

URL

http://pubs.acs.org/doi/full/10.1021/nl500733g

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=81497&p_lang=2

Resource files

File name	Size (MB)	Format
nl500733g.pdf	3,89	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Yakovlev Aleksey Valerevich	ru_RU
dc.date.accessioned	2014-01-01T00:00:00Z	ru_RU
dc.date.available	2014-01-01T00:00:00Z	ru_RU
dc.date.issued	2014	ru_RU
dc.identifier.citation	Alsu I. Zamaleeva, Mayeul Collot, Eloi Bahembera, Céline Tisseyre, Philippe Rostaing, Aleksey V. Yakovlev, Martin Oheim, Michel de Waard, Jean-Maurice Mallet, and Anne Feltz Nano Letters 2014 14 (6), 2994-3001 DOI: 10.1021/nl500733g	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=81497&p_lang=2	ru_RU
dc.description.abstract	Nano Letters	ru_RU
dc.description.abstract	Small-molecule chemical calcium (Ca2+) indicators are invaluable tools for studying intracellular signaling pathways but have severe shortcomings for detecting local Ca2+ entry. Nanobiosensors incorporating functionalized quantum dots (QDs) have emerged as promising alternatives but their intracellular use remains a major challenge. We designed cell-penetrating FRET-based Ca2+ nanobiosensors for the detection of local Ca2+ concentration transients, using commercially available CANdot565QD as a donor and CaRuby, a custom red-emitting Ca2+ indicator, as an acceptor. With Ca2+-binding affinities covering the range of 3?20 μM, our CaRubies allow building sensors with a scalable affinity for detecting intracellular Ca2+ transients at various concentrations. To facilitate their cytoplasmic delivery, QDs were further functionalized with a small cell-penetrating peptide (CPP) derived from hadrucalcin (HadUF1?11: H11), a ryanodine receptor-directed scorpion toxin identified within the venom of	ru_RU
dc.language.iso	ru	ru_RU
dc.subject		ru_RU
dc.title	Cell-Penetrating Nanobiosensors for Pointillistic Intracellular Ca2+-Transient Detection	ru_RU
dc.type	Articles in international journals and collections	ru_RU