KFU. Card publication. C-Terminus of Transcription Factor TnrA from Bacillus subtilis Controls DNA-Binding Domain Activity but Is Not Required for Dimerization.

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C-TERMINUS OF TRANS&SHY;CRIPTION FACTOR TNRA FROM BACILLUS SUBTILIS CONTROLS DNA-BINDING DOMAIN ACTIVITY BUT IS NOT REQUIRED FOR DIMERIZATION.

Form of presentation

Articles in international journals and collections

Year of publication

2013

Authors, employees of KFU

Ilinskaya Olga Nikolaevna, author

Kayumov Ayrat Rashitovich, author

Fedorova Kseniya Pavlovna, author

Other authors

M.I. Bogachev; E.Y. Turbina

Bibliographic description in the original language

K.P. Fedorova, I.S. Scharafutdinov, E.Y. Turbina, M.I. Bogachev, O.N. Ilinskaja, A.R. Kayumov Terminus of Transcription Factor TnrA from Bacillus subtilis Controls DNA-Binding Domain Activity but Is Not Required for Dimerization // Molecular Biology. - 2013. - V. 47. - � 2, R. 293–298.

Annotation

The transcription factor TnrA, which belongs to the MerR transcription regulators, in Bacillus subtilis controls genes of nitrogen metabolism during nitrogen limiting conditions. As all the DNA-binding proteins, it is active as a dimer in cells, but the dimerization site is still unknown. The multiple alignment of TnrA homologs from other Bacilli allowed to identify the putative dimerization sites. Using the C-terminal truncated TnrA proteins it is established, that, in contrast to other MerR-proteins, the TnrA C-terminus does not participate in dimerization. Surface plasmon resonance has revealed that C-terminus truncations of TnrA do not inactivate its DNA-binding activity. Contrary, increased the affinity towards DNA, confirming that C- terminus controls the DNA-binding activity in a full-length TnrA.

Keywords

Bacillus subtilis, transcription factor TnrA, dimerization.

The name of the journal

MOL BIOL REP

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=90440&p_lang=2

Resource files

File name	Size (MB)	Format
Molecular_Biology_2013.pdf	0,17	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Ilinskaya Olga Nikolaevna	ru_RU
dc.contributor.author	Kayumov Ayrat Rashitovich	ru_RU
dc.contributor.author	Fedorova Kseniya Pavlovna	ru_RU
dc.date.accessioned	2013-01-01T00:00:00Z	ru_RU
dc.date.available	2013-01-01T00:00:00Z	ru_RU
dc.date.issued	2013	ru_RU
dc.identifier.citation	K.P. Fedorova, I.S. Scharafutdinov, E.Y. Turbina, M.I. Bogachev, O.N. Ilinskaja, A.R. Kayumov Terminus of Transcription Factor TnrA from Bacillus subtilis Controls DNA-Binding Domain Activity but Is Not Required for Dimerization // Molecular Biology. - 2013. - V. 47. - � 2, �. 293–298.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=90440&p_lang=2	ru_RU
dc.description.abstract	MOL BIOL REP	ru_RU
dc.description.abstract	The transcription factor TnrA, which belongs to the MerR transcription regulators, in Bacillus subtilis controls genes of nitrogen metabolism during nitrogen limiting conditions. As all the DNA-binding proteins, it is active as a dimer in cells, but the dimerization site is still unknown. The multiple alignment of TnrA homologs from other Bacilli allowed to identify the putative dimerization sites. Using the C-terminal truncated TnrA proteins it is established, that, in contrast to other MerR-proteins, the TnrA C-terminus does not participate in dimerization. Surface plasmon resonance has revealed that C-terminus truncations of TnrA do not inactivate its DNA-binding activity. Contrary, increased the affinity towards DNA, confirming that C- terminus controls the DNA-binding activity in a full-length TnrA.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Bacillus subtilis	ru_RU
dc.subject	transcription factor TnrA	ru_RU
dc.subject	dimerization.	ru_RU
dc.title	C-Terminus of Transcription Factor TnrA from Bacillus subtilis Controls DNA-Binding Domain Activity but Is Not Required for Dimerization.	ru_RU
dc.type	Articles in international journals and collections	ru_RU