KFU. Card publication. Conjugates of a photoactivated rhodamine with biopolymers for cell staining

KAZAN
FEDERAL UNIVERSITY

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CONJUGATES OF A PHOTOACTIVATED RHODAMINE WITH BIOPOLYMERS FOR CELL STAINING

Form of presentation

Articles in international journals and collections

Year of publication

2014

Язык

английский

Authors, employees of KFU

Soloveva Valeriya Vladimirovna, author

Bibliographic description in the original language

Zaitsev S.Y. Conjugates of a photoactivated rhodamine with biopolymers for cell staining / S.Y. Zaitsev, M.N. Shaposhnikov, D.O. Solovyeva, V.V. Solovyeva, A.A. Rizvanov // The Scientific World Journal. – 2014. – V.2014. - Article ID 285405. – doi: 10.1155/2014/285405.

Annotation

Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan (?Chitosan-PFD?) and histone H1 (?Histone H1.3-PFD?). The intensive fluorescence has been observed after photoactivation of these conjugates inside cells (A431, HaCaT, HEK239, HBL-100, and MDCK). Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes (?caged? dyes) for microscopic probing of biological objects. Thus, the synthesized ?Chitosan-PFD? and ?Histone H1-PFD? have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy.

Keywords

photoactivated rhodamine dyes, histone H1.3

The name of the journal

The scientific world journal

URL

http://www.hindawi.com/journals/tswj/2014/285405/

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=93684&p_lang=2

Full metadata record

Field DC	Value	Language
dc.contributor.author	Soloveva Valeriya Vladimirovna	ru_RU
dc.date.accessioned	2014-01-01T00:00:00Z	ru_RU
dc.date.available	2014-01-01T00:00:00Z	ru_RU
dc.date.issued	2014	ru_RU
dc.identifier.citation	Zaitsev S.Y. Conjugates of a photoactivated rhodamine with biopolymers for cell staining / S.Y. Zaitsev, M.N. Shaposhnikov, D.O. Solovyeva, V.V. Solovyeva, A.A. Rizvanov // The Scientific World Journal. – 2014. – V.2014. - Article ID 285405. – doi: 10.1155/2014/285405.	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=93684&p_lang=2	ru_RU
dc.description.abstract	The scientific world journal	ru_RU
dc.description.abstract	Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan (?Chitosan-PFD?) and histone H1 (?Histone H1.3-PFD?). The intensive fluorescence has been observed after photoactivation of these conjugates inside cells (A431, HaCaT, HEK239, HBL-100, and MDCK). Developed procedures and obtained data are important for further application of novel precursors of fluorescent dyes (?caged? dyes) for microscopic probing of biological objects. Thus, the synthesized ?Chitosan-PFD? and ?Histone H1-PFD? have been successfully applied in this study for intracellular transport visualization by fluorescent microscopy.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	photoactivated rhodamine dyes	ru_RU
dc.subject	histone H1.3	ru_RU
dc.title	Conjugates of a photoactivated rhodamine with biopolymers for cell staining	ru_RU
dc.type	Articles in international journals and collections	ru_RU