KFU. Card publication. Determination of ROS Generation Rates in Plant Mitochondria in vitro Using Fluorescent Indicators: Non-Specific Effects of Inhibitors of Terminal Oxidases

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DETERMINATION OF ROS GENERATION RATES IN PLANT MITOCHONDRIA IN VITRO USING FLUORESCENT INDICATORS: NON-SPECIFIC EFFECTS OF INHIBITORS OF TERMINAL OXIDASES

Form of presentation

Articles in Russian journals and collections

Year of publication

2015

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Authors, employees of KFU

Abdrakhimova Yoldyz Raisovna, author

Other authors

Andreev Igor Mikhaylovich, author

Shugaev Aleksandr Grigorevich, author

Bibliographic description in the original language

Abdrakhimova Y.R., I.M.Andreev,A.G.Shugaev.Determination of ROS Generation Rates in Plant Mitochondria in vitro Using Fluorescent Indicators: Non-Specific Effects of Inhibitors of Terminal Oxidases//Russian Journal of Plant Physiology, 2015, Vol. 62, No. 1, pp. 136-141. DOI: 10.1134/S1021443714060028

Annotation

A possibility of quantitative analysis of the ROS production by mitochondria isolated from etiolated winter wheat seedlings was studied by following hydrogen peroxide accumulation in medium of organelle incubation using a new highly sensitive test-system including the fluorogenic indicator Amplex Red and horseradish peroxidase. Experimental evidence is presented indicating that the classical inhibitors of the terminal oxidases of the plant mitochondrial respiratory chain can inactivate the ROS detecting test-systems in vitro because of interactions with their functional components. These results are discussed in comparison with literature data obtained by similar test-systems and indicate that there are considerable limitations for obtaining reliable experimental evidence of the antioxidant role of alternative cyanide-resistant oxidase in plant mitochondria.

Keywords

Triticum aestivum, mitochondria, reactive oxygen species, fluorescent indicators, Amplex Red, alamethicin

The name of the journal

Russian Journal of Plant Physiology

URL

http://link.springer.com/article/10.1134/S1021443714060028

Please use this ID to quote from or refer to the card

https://repository.kpfu.ru/eng/?p_id=98432&p_lang=2

Resource files

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AbdrakhimovaRJPP_15.pdf	0,22	pdf	show / download
AbdrakhimovaRJPP_15.pdf	0,22	pdf	show / download

Full metadata record

Field DC	Value	Language
dc.contributor.author	Abdrakhimova Yoldyz Raisovna	ru_RU
dc.contributor.author	Andreev Igor Mikhaylovich	ru_RU
dc.contributor.author	Shugaev Aleksandr Grigorevich	ru_RU
dc.date.accessioned	2015-01-01T00:00:00Z	ru_RU
dc.date.available	2015-01-01T00:00:00Z	ru_RU
dc.date.issued	2015	ru_RU
dc.identifier.citation	Abdrakhimova Y.R., I.M.Andreev,A.G.Shugaev.Determination of ROS Generation Rates in Plant Mitochondria in vitro Using Fluorescent Indicators: Non-Specific Effects of Inhibitors of Terminal Oxidases//Russian Journal of Plant Physiology, 2015, Vol. 62, No. 1, pp. 136-141. DOI: 10.1134/S1021443714060028	ru_RU
dc.identifier.uri	https://repository.kpfu.ru/eng/?p_id=98432&p_lang=2	ru_RU
dc.description.abstract	Russian Journal of Plant Physiology	ru_RU
dc.description.abstract	A possibility of quantitative analysis of the ROS production by mitochondria isolated from etiolated winter wheat seedlings was studied by following hydrogen peroxide accumulation in medium of organelle incubation using a new highly sensitive test-system including the fluorogenic indicator Amplex Red and horseradish peroxidase. Experimental evidence is presented indicating that the classical inhibitors of the terminal oxidases of the plant mitochondrial respiratory chain can inactivate the ROS detecting test-systems in vitro because of interactions with their functional components. These results are discussed in comparison with literature data obtained by similar test-systems and indicate that there are considerable limitations for obtaining reliable experimental evidence of the antioxidant role of alternative cyanide-resistant oxidase in plant mitochondria.	ru_RU
dc.language.iso	ru	ru_RU
dc.subject	Triticum aestivum	ru_RU
dc.subject	mitochondria	ru_RU
dc.subject	reactive oxygen species	ru_RU
dc.subject	fluorescent indicators	ru_RU
dc.subject	Amplex Red	ru_RU
dc.subject	alamethicin	ru_RU
dc.title	Determination of ROS Generation Rates in Plant Mitochondria in vitro Using Fluorescent Indicators: Non-Specific Effects of Inhibitors of Terminal Oxidases	ru_RU
dc.type	Articles in Russian journals and collections	ru_RU